| 高效液相色谱-串联质谱内标法同时测定水产品中15种喹酮类药物残留量 |
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| 引用本文： | 王志杰,冷凯良,孙伟红,宁劲松,翟毓秀,谭志军,郭萌萌.高效液相色谱-串联质谱内标法同时测定水产品中15种喹酮类药物残留量[J].分析科学学报,2010,26(4). |
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| 作者姓名： | 王志杰 冷凯良 孙伟红 宁劲松 翟毓秀 谭志军 郭萌萌 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所,山东青岛,266071 |
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| 基金项目： | 国家863计划，农业部行业专项，基本科研业务费 |
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| 摘 要： | 建立了水产品中15种喹诺酮类药物(QNs)残留量的高效液相色谱-串联质谱(HPLC-MS/MS)测定方法。以氘代试剂为内标,样品经酸性乙腈萃取后,用正己烷脱脂,旋转蒸发浓缩,采用HPLC-MS/MS选择反应监测(SRM)正离子模式测定,内标法定量。可同时对水产品中的15种QNs进行定性和定量测定。15种QNs的检出限(S/N=3)为1.0μg/kg,定量限(S/N=10)为2.0μg/kg;在10.0～200.0ng/mL时峰强度与质量浓度的线性关系良好(r0.99)。方法的平均回收率范围为66%～121%。该法简便快捷,分析成本低,在一定程度上实现了药物残留的快速检测。
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| 关 键 词： | 高效液相色谱-串联质谱 内标法 水产品 喹诺酮类药物 多残留同时测定 |
Simultaneous Determination of 15 Quinolone Residues in Aquatic Products by HPLC-MS/MS with Internal Standard Method |
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| WANG Zhi-jie,LENG Kai-liang,SUN Wei-hong,NING Jin-song,ZHAI Yu-xiu,TAN Zhi-jun,GUO Meng-meng.Simultaneous Determination of 15 Quinolone Residues in Aquatic Products by HPLC-MS/MS with Internal Standard Method[J].Journal of Analytical Science,2010,26(4). |
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| Authors: | WANG Zhi-jie LENG Kai-liang SUN Wei-hong NING Jin-song ZHAI Yu-xiu TAN Zhi-jun GUO Meng-meng |
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| Institution: | WANG Zhi-jie,LENG Kai-liang,SUN Wei-hong,NING Jin-song,ZHAI Yu-xiu,TAN Zhi-jun,GUO Meng-meng(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071) |
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| Abstract: | A method for simultaneous determination of 15 QNs residues in aquatic products was developed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Deuterium substituted reagents were added to the sample before extraction as internal standard. The sample was extracted with acidified acetonitril,cleaned-up with hexane,and concentrated with a rotary evaporator.The mass spectrometer was operated in positive ion mode using select reaction monitoring for qualitative and quantitative ana... |
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| Keywords: | High performance liquid chromatography-tandem mass spectrometry Internal standard method Aquatic products Quinolone Multi-residue Simultaneous determination |
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