反胶束萃取-高效液相色谱法测定磺酸型偶氮染料

建立了水中磺酸偶氮染料甲基橙（MO）、刚果红（CR）和酸性铬兰K（ACBK）的反胶束萃取-离子对高效液相色谱定量检测的方法。采用Hypersil C18柱（250×4．6mm，5μm），流动相为V（甲醇）／V（水）=63：37（含10mmol／L的KH2PO4、4mmol／L的四丁基溴化铵，KOH调pH=7．0），流速为0.8mL／min，MO、CR和ACBK检测波长分别为449nm、505nm和526nm。结果表明，染料的回收率为92．9％～102．1％，相对标准偏差为0．9％～2．3％，水中MO、CR和ACBK的检出限分别为0．6μg／L、1．2μg／L和1．3μg／L。

Reversed Micelles Extraction and HPLC Determinnation of the Sulfonic Acid Azodyes

A ion-pair high performance liquid chromatographic (RP-HPLC) method was established for the determination of sulfonic acid azodye including methyl orange (MO), congo red (CR) and acid chrome blue K (ACBK) extracted using reverse micelles from aqueous samples. A Hypersil C18 column (250×4.6 mm,5 μm) mmol/L phosphate buffer and 4 mmol/L tetrabutylammonium bromide as the mobile phase. The flow rate of mobile phase was 0.8 mL/min, and the detection wavelength was 449 nm for MO, 505 nm for CR and 526 nm for ACBK, respectively. The results showed the recoveries and relative standard deviations of the azodyes were 92.9% ～ 102.1% and 0.9%～2.3%, and the detection limits of MO, CR and ACBK in water were 0.6, 1.2 and 1.3 μg/L, respectively.