| 八肋游仆虫中心蛋白N-端半分子与Tb3+，Ca2+的结合 |
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| 引用本文： | 任列香,赵亚琴,丰九英,贺晓静,梁爱华,杨斌盛.八肋游仆虫中心蛋白N-端半分子与Tb3+，Ca2+的结合[J].无机化学学报,2006,22(1):87-90. |
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| 作者姓名： | 任列香 赵亚琴 丰九英 贺晓静 梁爱华 杨斌盛 |
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| 作者单位： | 山西大学分子科学研究所,化学生物学与分子工程教育部重点实验室,太原,030006 |
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| 基金项目： | 中国科学院资助项目;山西省自然科学基金 |
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| 摘 要： | 用分子生物学方法表达、纯化了游仆虫中心蛋白及N-端半分子，用铽荧光探针法、离子竞争法研究了pH 7.4，0.01 mol·
L-1 Hepes条件下中心蛋白与铽、钙的结合性质。结果表明中心蛋白有4个铽结合部位，其中2个为高亲合结合部位、2个为低亲合结合部位。具有2个低亲合结合部位的中心蛋白半分子与铽结合的条件常数是(2.13±0.10)×105 L·mol-1，与钙结合的条件常数是(7.52±0.02)×102 L·mol-1。
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| 关 键 词： | 中心蛋白 荧光 铽离子 钙离子 |
| 文章编号： | 1001-4861(2006)01-0087-04 |
| 收稿时间： | 2005-07-08 |
| 修稿时间： | 2005-10-24 |
Terbium- and Calcium-binding Properties of N-terminal Domain of Euplotes Centrin |
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| REN Lie-Xiang,ZHAO Ya-Qin,FENG Jiu-Ying,HE Xiao-Jing,LIANG Ai-Hua and YANG Bin-Sheng.Terbium- and Calcium-binding Properties of N-terminal Domain of Euplotes Centrin[J].Chinese Journal of Inorganic Chemistry,2006,22(1):87-90. |
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| Authors: | REN Lie-Xiang ZHAO Ya-Qin FENG Jiu-Ying HE Xiao-Jing LIANG Ai-Hua and YANG Bin-Sheng |
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| Institution: | Institute of Molecular Science, Key for Laboratory Chemical Biology and Molecular Engineering, Education of Ministry, Shanxi University, Taiyuan 030006,Institute of Molecular Science, Key for Laboratory Chemical Biology and Molecular Engineering, Education of Ministry, Shanxi University, Taiyuan 030006,Institute of Molecular Science, Key for Laboratory Chemical Biology and Molecular Engineering, Education of Ministry, Shanxi University, Taiyuan 030006,Institute of Molecular Science, Key for Laboratory Chemical Biology and Molecular Engineering, Education of Ministry, Shanxi University, Taiyuan 030006,Institute of Molecular Science, Key for Laboratory Chemical Biology and Molecular Engineering, Education of Ministry, Shanxi University, Taiyuan 030006 and Institute of Molecular Science, Key for Laboratory Chemical Biology and Molecular Engineering, Education of Ministry, Shanxi University, Taiyuan 030006 |
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| Abstract: | Euplotes centrins (EoCens) including full-length protein (apoEoCen) and semi-molecule centrin (N-apoEoCen) were expressed and purified by biological engineering. The binding of terbium
(Tb3+) to apoEoCen was monitored by fluorescence in 0.01 mol·L-1 N-2-hydro-xyethylpiperazine-N-2-ethanesulfonic acid (Hepes), at pH 7.4. It could be seen that the protein binds two
Tb3+ with high affinity and two Tb3+ with low affinity. The reactions between N-apoEoCen and
Tb3+ or Ca2+ were also studied by Tb3+ fluorescence probe and ionic competition in 0.01 mol·L-1 Hepes, at pH 7.4. On the basis of fluorescence titration curves, the conditional binding constant of
Tb2-N-EoCen was determined to be (2.13 ± 0.10) × 105 L·mol-1 and the relative binding constant of
Ca2-N-EoCen was calculated to be (7.52 ± 0.02) × 102 L·mol-1. |
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| Keywords: | Euplotes centrin fluorescence Tb3+ Ca2+ |
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