| Raman光谱研究铜锌超氧化物歧化酶及其金属取代衍生物的二级结构 |
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| 引用本文： | 汤银燕,王志林,郁清,耿金龙,罗勤慧.Raman光谱研究铜锌超氧化物歧化酶及其金属取代衍生物的二级结构[J].无机化学学报,2001,17(2):263-268. |
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| 作者姓名： | 汤银燕 王志林 郁清 耿金龙 罗勤慧 |
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| 作者单位： | 南京大学配位化学研究所, |
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| 基金项目： | 国家自然科学基金资助项目(No.29831010)。 |
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| 摘 要： | 本文测定了铜锌超氧化物歧化酶（Cu2Zn2SOD)及其金属取代衍生物Cu2Ni2SOD的Raman光谱，对图谱进行了归属，并定量测定了两种SOD的二级结构，同时对结构与活性的关系进行了讨论。
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| 关 键 词： | 铜锌超氧化物歧化酶 Raman光谱 二级结构 金属取代衍生物 活性 |
| 收稿时间： | 2000/11/8 0:00:00 |
| 修稿时间： | 2000年11月8日 |
A Study on the Secondary Structures of Copper-Zinc Superokide Dismutase and its Metal-Substituted Derivatives by Raman Spectroscopy |
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| TANG Yin-Yan,WANG Zhi-Lin,YU Qing,GENG Jin-Long and LUO Qin-Hui.A Study on the Secondary Structures of Copper-Zinc Superokide Dismutase and its Metal-Substituted Derivatives by Raman Spectroscopy[J].Chinese Journal of Inorganic Chemistry,2001,17(2):263-268. |
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| Authors: | TANG Yin-Yan WANG Zhi-Lin YU Qing GENG Jin-Long and LUO Qin-Hui |
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| Institution: | State Key Laboratory of Coordination Chemistry Nanjing University, Nanjing 210093,State Key Laboratory of Coordination Chemistry Nanjing University, Nanjing 210093,State Key Laboratory of Coordination Chemistry Nanjing University, Nanjing 210093,State Key Laboratory of Coordination Chemistry Nanjing University, Nanjing 210093 and State Key Laboratory of Coordination Chemistry Nanjing University, Nanjing 210093 |
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| Abstract: | The Raman spectra of copper-zinc superoxide dismutase(Cu2Zn2SOD) and its metal-substituted derivative Cu2Ni2SOD were measured and the major bands were assigned. The secondary structure contents of the proteins were obtained quantitatively. The results show that the Cu2Zn2SOD contains 3.0% α-helix, 45.1% β-structure and 51.9% random coil, which is in agreement with the results obtained by the X-ray crystal analysis and FTIR, and the Cu2Ni2SOD contains 8.2% α-helix,27.2% β-structure and 64.1% random coil. It can be concluded that the activity of SOD decreases with the decreasing of β-structure and increasing of random coil. |
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| Keywords: | superoxide dismutae Raman spectroscopy secondary structure |
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