两个多胺铜配合物的合成，晶体结构以及DNA切割活性

本文以配体N,N′-二(3-氨丙基)-4-甲氧基苄胺(amba)与4,4′-联吡啶,铜盐反应合成了两种多胺Cu(Ⅱ)配合物Cu(amba)Cl2](1)和Cu(4,4′-bipy)(amba)(ClO4)]ClO4(2),通过红外光谱、质谱和X-射线单晶衍射对其结构进行了表征。利用紫外吸收光谱、分子荧光,电化学以及粘度试验研究了配合物与小牛胸腺DNA(CT-DNA)的相互作用方式。通过紫外吸收光谱得到配合物1和2与DNA的结合常数Kb分别为1.3×104 mol-1.L和1.7×104 mol-1.L,荧光光谱得到配合物1和2的荧光淬灭常数分别为1.04×103mol-1.L和1.81×103 mol-1.L,表明了配合物1和2与CT-DNA结合方式均为静电模式。凝胶电泳实验的结果表明配合物对pBR322 DNA的切割均为水解切割。

Synthesis,Crystal Structure,and DNA Cleavage Activity of Two Polyamine Copper(Ⅱ) Complexes

Two functional polyamine copper(Ⅱ) complexes,Cu(amba)Cl2](1) and Cu(4,4′-bipy)(amba)(ClO4)]ClO4(2),have been obtained easily via evaporation of the mixture of N,N-bis(3-aminopropyl)-4-methoxybenzylamine(amba) and 4,4′-bipy and cupric salts.The complexes were characterized by IR,ES-MS and X-ray single crystal diffraction techniques.The interactions of the complexes with calf thymus DNA(CT-DNA) have been measured by UV-Vis absorption,fluorescent spectroscopy,electrochemical experiment and viscosity experiment.Absorption spectroscopic investigation reveals that the calculated binding constants(Kb) values for the two complexes are 1.3×104 mol-1.L for 1 and 1.7×104 mol-1.L for 2,respectively.Fluorescence spectroscopy shows that Stern-Volmer quenching constants of complexes 1 and 2 are 1.04×103 mol-1.L and 1.81×103 mol-1.L,respectively.The results indicate that the binding modes of the two complexes interact with DNA may be not intercalative modes but electrostatic modes.Moreover,the higher constant value of 2 shows the interaction of 2 with DNA is stronger than that of 1.The agarose gel electrophoresis studies reveal that both the complexes cleave pBR322 DNA via a hydrolytic pathway.