| pH值和钙离子对尖吻蝮蛇抗血小板凝集素二级结构的影响 |
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| 引用本文： | 陈结霞,徐小龙,王守业,张立云,刘清亮.pH值和钙离子对尖吻蝮蛇抗血小板凝集素二级结构的影响[J].无机化学学报,2007,23(5):849-853. |
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| 作者姓名： | 陈结霞 徐小龙 王守业 张立云 刘清亮 |
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| 作者单位： | 中国科学技术大学化学系,合肥,230026 |
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| 摘 要： | 尖吻蝮蛇毒中抗血小板凝集素是凝血因子IX/凝血因子X结合蛋白，它具有抗凝血和抑制血小板凝集双重活性。用红外光谱、拉曼光谱和CD谱研究了抗血小板凝集素的二级结构以及pH值和钙离子对其二级结构的影响。用CD谱测得，在水溶液中，抗血小板凝集素的主要骨架构象为β-折叠(26.3%)和α-螺旋(19.6%)结构。拉曼光谱显示，在粉末状态，其α-螺旋含量显著降低。CD谱还表明，抗血小板凝集素在pH值3.0～11.0范围内保持稳定的天然结构，钙离子诱导的抗血小板凝集素结构变化是可逆的，钙离子在稳定抗血小板凝集素的天然结构中起重要作用。
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| 关 键 词： | 抗血小板凝集素 二级结构 圆二色谱 拉曼光谱 红外光谱 |
| 文章编号： | 1001-4861(2007)05-0849-05 |
| 修稿时间： | 2007-01-22 |
Effects of pH Value and Ca2+ Ion on the Secondary Structure of Agkisacutacin from Agkistrodon Acutus Venom |
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| CHEN Jie-Xi,XU Xiao-Long,WANG Shou-Ye,ZHANG Li-Yun and LIU Qing-Liang.Effects of pH Value and Ca2+ Ion on the Secondary Structure of Agkisacutacin from Agkistrodon Acutus Venom[J].Chinese Journal of Inorganic Chemistry,2007,23(5):849-853. |
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| Authors: | CHEN Jie-Xi XU Xiao-Long WANG Shou-Ye ZHANG Li-Yun and LIU Qing-Liang |
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| Institution: | Department of Chemistry, University of Science and Technology of China, Hefei 230026,Department of Chemistry, University of Science and Technology of China, Hefei 230026,Department of Chemistry, University of Science and Technology of China, Hefei 230026,Department of Chemistry, University of Science and Technology of China, Hefei 230026 and Department of Chemistry, University of Science and Technology of China, Hefei 230026 |
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| Abstract: | Agkisacutacin isolated from the venom of Agkistrodon acutus is a coagulation factor Ⅸ/coagulation factor Ⅹ-binding protein with marked anticoagulant- and platelet-modulating activities. The effects of pH value and Ca2+ ion on the secondary structure of Agkisacutacin have been studied by FTIR, Raman and circular dichroism (CD) spectroscopy. The main backbone conformations of holo-Agkisacutacin are α-helix (19.6%) and β-sheet (26.3%) as determined by CD spectroscopy. According to the Raman measurements, the content of α-helix of holo-Agkisacutacin in solid powder is less than that in solution. The conformation of Agkisacutacin is stable in the pH range of 3.0～11.0 as shown by CD spectroscopy. Ca2+-induced conformation change of Agkisacutacin is reversible. Ca2+ ion plays an important role in the stabilization of the structure of Agkisacutacin. |
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| Keywords: | agkisacutacin secondary structure circular dichroism Raman Resonance FTIR |
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