| 硝酸锶对原代培养的小鼠成骨细胞增殖、分化和矿化功能的影响 |
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| 引用本文： | 张金超,郝晓红,张 群,李亚平,王书香.硝酸锶对原代培养的小鼠成骨细胞增殖、分化和矿化功能的影响[J].无机化学学报,2012,28(2):374-380. |
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| 作者姓名： | 张金超 郝晓红 张 群 李亚平 王书香 |
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| 作者单位： | 河北大学化学与环境科学学院,河北省化学生物学重点实验室,保定071002 |
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| 基金项目： | 国家自然科学基金，河北省自然科学基金重点 |
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| 摘 要： | 采用噻唑蓝(MTT)法、碱性磷酸酶(ALP)比活性测定、油红O染色、Ⅰ型胶原测定以及矿化结节染色及定量分析等方法,研究了不同浓度的硝酸锶对原代培养的成骨细胞增殖、分化、矿化功能以及横向分化为脂肪细胞的影响。结果表明:硝酸锶对成骨细胞增殖、分化、矿化功能以及横向分化为脂肪细胞的影响与作用浓度和时间密切相关,但没有呈现出剂量依赖性。结果提示,硝酸锶对骨代谢的影响是复杂的,其具有保护还是损害作用取决于作用浓度和时间,而且它们是影响硝酸锶生物效应(从损伤到保护)转变的关键因素。
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| 关 键 词： | 硝酸锶 成骨细胞 增殖 分化 矿化 |
Effects of Strontium Nitrate on the Proliferation, Differentiation and Mineralization Function of Primary Mouse Osteoblasts in vitro |
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| ZHANG Jin-Chao,HAO Xiao-Hong,ZHANG Qun,LI Ya-Ping and WANG Shu-Xiang.Effects of Strontium Nitrate on the Proliferation, Differentiation and Mineralization Function of Primary Mouse Osteoblasts in vitro[J].Chinese Journal of Inorganic Chemistry,2012,28(2):374-380. |
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| Authors: | ZHANG Jin-Chao HAO Xiao-Hong ZHANG Qun LI Ya-Ping and WANG Shu-Xiang |
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| Institution: | College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, Hebei 071002, China,College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, Hebei 071002, China,College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, Hebei 071002, China,College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, Hebei 071002, China and College of Chemistry & Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding, Hebei 071002, China |
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| Abstract: | The effect of Sr(NO3)2 concentration on proliferation,differentiation and mieralizerzation function of primary osteblasts(OBs) in vitro was evaluated by using the 3-(4,5-dimethyl)-2,5-diphenyl tetra-zolium bromide(MTT)method,alkaline phosphatase(ALP) activity,oil red assays,quantitative analysis of promotion rate for type Ⅰ collagen production and Alizarin Red Stain(ARS).The results indicate that the effect of Sr(NO3)2 on the proliferation,differentiation,mieralizerzation function and adipocytic transdifferentiation of OBs depends on the concentration and incubation time,however,the effect is independent of dosage.This suggests the effect of Sr(NO3)2 on bone metabolism is a complex issue.Whether the effect of Sr(NO3)2 is damaging or protection is dependent on two key factors,i.e.,the concentration and culture time. |
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| Keywords: | strontium nitrate osteoblasts proliferation differentiation mineralization |
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