载药脂质体复合缺钙磷灰石骨水泥支架的研究

采用食盐颗粒浸出法制备了缺钙磷灰石水泥(CPC)多孔支架;用脂质体包裹盐酸万古霉素制备了载药脂质体。将它们两者结合,制备了脂质体载药复合缺钙磷灰石水泥(dl-CPC)支架。结果表明:缺钙CPC多孔支架能够将载药脂质体吸附在其大孔表面或微孔里;dl-CPC支架对MC3T3-E1细胞的生长没有负面影响,显示出良好的细胞相容性。此外,dl-CPC支架具有很好的抗菌性能,能够抑制大肠杆菌生长,抗菌率达99%(12 h)。dl-CPC支架浸泡在磷酸缓冲溶液中,释放药物的速度比较缓慢(前4周);而直接吸附药物的CPC支架,在1周内大部分药物释放出来,出现暴释现象。另结果表明:dl-CPC支架具有缓释药物和骨再生的双重功能,可用于骨缺损的修复及治疗慢性骨髓炎。

Composite Scaffold of Liposome Loaded Drug and Calcium Deficient Apatite Cement

Calcium deficient apatite cement (CPC) scaffold was prepared by using salt particle leach method, liposome loaded drug (Vancomycin hydrochloride) was also fabricated. Composite scaffold of liposome loaded drug and calcium deficient apatite cement (dl-CPC) was developed by combination of both them. The results showed that the calcium deficient CPC could adsorb liposome loaded drug on both the wall of the macropores and micropores of the scaffold. The dl-CPC scaffold loaded drug had no negative effects on MC3T3-E1 cells growth, showing good cytocompatiblity. In addition, the dl-CPC scaffold had good antibacterial properties, which could inhibit the growth of E. coli, and the antibacterial ratio could reach 99% after incubated for 12 hours. The drug was slowly released from the dl-CPC scaffold l (in the first 4 weeks), when the dl-CPC soaked into in phosphate buffer solution (PBS). However, waterfall release of drug was found that the great mass of drug was released from the CPC scaffold directly loaded drug in the first week. The results suggested that the dl-CPC scaffold had two functions of bone regeneration and drug slow release, which can be used for bone defects repair and therapy of chronic osteomyelitis.