| 吡咯腙对溶酶体中Hg2+的荧光成像 |
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| 引用本文： | 王震,李思媛,王元,吴伟娜,张磊,陈忠,闫玲玲.吡咯腙对溶酶体中Hg2+的荧光成像[J].无机化学学报,2023,39(6):1122-1130. |
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| 作者姓名： | 王震 李思媛 王元 吴伟娜 张磊 陈忠 闫玲玲 |
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| 作者单位： | 河南理工大学化学化工学院, 焦作 454000;江西科技师范大学材料与机电学院, 南昌 330013;河南理工大学物理与电子信息学院, 焦作 454000 |
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| 基金项目： | 国家自然科学基金(No.21907023，21001040，61964008)、河南省重点研发与推广专项科技攻关项目(No.232102320092)、江西省自然科学基金(No.20212BAB204017)、河南省高校基本科研业务费项目(No.NSFRF230402)、河南理工大学“双一流”创建工程项目(No.AQ20230745，AQ20230754)和河南理工大学研究生教改项目(No.2021YJ16)资助。 |
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| 摘 要： | 合成了一种新的吡咯腙探针1,用于Hg2+的比色和荧光开启检测。探针1对Hg2+的检测限为45 nmol·L-1,缔合常数为5.78×108 L·mol-1。值得注意的是,工作pH范围为4.0~10.0。Job曲线和MS数据证实探针与Hg2+形成1:1的配合物。通过1H NMR、时间分辨荧光光谱和密度泛函理论(DFT)计算系统研究了探针与Hg2+的配位模式。此外,由于吗啉基团的存在,探针可以检测HeLa细胞溶酶体中的Hg2+。
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| 关 键 词： | 细胞成像 荧光探针 Hg2+ 腙 溶酶体 吡咯 |
| 收稿时间： | 2022/4/3 0:00:00 |
| 修稿时间： | 2023/4/27 0:00:00 |
Pyrrole-based hydrazone for fluorescent imaging of Hg2+ in lysosomes |
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| WANG Zhen,LI Si-Yuan,WANG Yuan,WU Wei-N,ZHANG Lei,CHEN Zhong,YAN Ling-Ling.Pyrrole-based hydrazone for fluorescent imaging of Hg2+ in lysosomes[J].Chinese Journal of Inorganic Chemistry,2023,39(6):1122-1130. |
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| Authors: | WANG Zhen LI Si-Yuan WANG Yuan WU Wei-N ZHANG Lei CHEN Zhong YAN Ling-Ling |
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| Institution: | College of Chemistry and Chemical Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China;School of Materials and Mechanical and Electrical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013 China; School of Physics and Electronic Information Engineering, Henan Polytechnic University, Jiaozuo, Henan 454000 China |
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| Abstract: | A novel pyrrole-based hydrazone has been synthesized for colorimetric and fluorescent turn-on detection of Hg2+ ions. A 1:1 binding ratio of the Hg2+ complex has been obtained from Job''s plots and MS data. The coordination mode was systematically investigated by 1H NMR, time-resolved fluorescence spectroscopy, and density functional theory (DFT) calculations. The limit of detection of 1 for Hg2+ was as low as 45 nmol·L-1 with an association constant of 5.78×108 L·mol-1. It is worth noting that the response of 1 to Hg2+ was good in a pH range of 4.0 to 10.0. In addition, owing to the existence of the morpholine group, the probe can detect lysosomal Hg2+ in HeLa cells. |
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| Keywords: | cell imaging fluorescent probe Hg2+ hydrazone lysosome pyrrole |
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