| 溶胶-凝胶法制备的二氧化硅中肌红蛋白的苯胺羟化酶活性 |
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| 引用本文： | 王威,罗雪梅,黄炜,王金晞,朱龙根.溶胶-凝胶法制备的二氧化硅中肌红蛋白的苯胺羟化酶活性[J].无机化学学报,1998,14(3):318-321. |
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| 作者姓名： | 王威 罗雪梅 黄炜 王金晞 朱龙根 |
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| 作者单位： | 南京大学配位化学国家重点实验室，配位化学研究所，南京 210093,南京大学配位化学国家重点实验室，配位化学研究所，南京 210093,南京大学配位化学国家重点实验室，配位化学研究所，南京 210093,南京大学配位化学国家重点实验室，配位化学研究所，南京 210093,南京大学配位化学国家重点实验室，配位化学研究所，南京 210093 |
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| 摘 要： | 用溶胶-凝胶制备了多孔透明的二氧化硅包埋肌红蛋白，在还原辅酶Ⅰ二钠盐(NADH)和黄素单核苷酸(FMN)的存在下，用可见光谱法测定了肌红蛋白苯胺羟基化反应的Km和kcat。表明包埋在二氧化硅中的肌红蛋白保持了苯胺羟化酶活性。
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| 关 键 词： | 溶胶-凝胶 肌红蛋白 苯胺羟化酶 |
ANILINE-HYDROXYLASE ACTIVITY OF MYOGLOBIN ENCAPSULATED IN SILICATE SOL-GELS |
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| Wang Wei,Luo Xuemei,Huang Wei,Wang Jinxi and Zhu Longgen.ANILINE-HYDROXYLASE ACTIVITY OF MYOGLOBIN ENCAPSULATED IN SILICATE SOL-GELS[J].Chinese Journal of Inorganic Chemistry,1998,14(3):318-321. |
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| Authors: | Wang Wei Luo Xuemei Huang Wei Wang Jinxi and Zhu Longgen |
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| Institution: | State Key Laboratory of Coordination Chemistry, Institute of Coordination Chemistry, Nanjing University, Nanjing 210093,State Key Laboratory of Coordination Chemistry, Institute of Coordination Chemistry, Nanjing University, Nanjing 210093,State Key Laboratory of Coordination Chemistry, Institute of Coordination Chemistry, Nanjing University, Nanjing 210093,State Key Laboratory of Coordination Chemistry, Institute of Coordination Chemistry, Nanjing University, Nanjing 210093 and State Key Laboratory of Coordination Chemistry, Institute of Coordination Chemistry, Nanjing University, Nanjing 210093 |
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| Abstract: | Using sol-gel procedures, we encapsulated myoglobin in the porous and transparent silicate glass. The transparency of sol-gel makes it feasible for us to monitor the hydroxylation reaction spectroscopically in the pore of the matrix. Thus the Km and kcat values of the hydroxylation reaction for Mb-sol-gel were determined to be 10.3 mmol·L-1 and 0.013 min-1, respectively. It shows that the aniline-hydroxylase activity of myoglobin (Mb) encapsulated in silicte sol-gels in maintained and can be induced by a semi-artificial multienzyme system of NADH-FMN-Mb. |
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| Keywords: | sol-gel myoglobin aniline-hydroxylase |
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