| 一种快速响应的线粒体靶向荧光探针用于检测活细胞和斑马鱼中的次氯酸盐 |
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| 引用本文： | 张长丽,张晶晶,沈优,陆嘉成,黄芳,徐莉.一种快速响应的线粒体靶向荧光探针用于检测活细胞和斑马鱼中的次氯酸盐[J].无机化学学报,2022,38(8):1623-1632. |
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| 作者姓名： | 张长丽 张晶晶 沈优 陆嘉成 黄芳 徐莉 |
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| 作者单位： | 南京晓庄学院环境科学学院, 南京市先进功能材料重点实验室, 南京 211171;南京林业大学理学院, 南京 210093 |
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| 基金项目： | 江苏高校青蓝工程项目和江苏省自然科学基金（No.BK20211013）资助 |
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| 摘 要： | 以香豆素为荧光团，设计并合成了一种线粒体靶向荧光探针Cou-Py，用于检测ClO-。由于肟基在激发态的C=N异构化，Cou-Py表现出非常微弱的荧光，能够实现ClO-促发的脱氧反应，在5 s内实现荧光恢复。此外，Cou-Py对ClO-表现出高的选择性和低的检测限（6.87 nmol·L-1）。重要的是，Cou-Py已成功用于检测MCF-7细胞线粒体和斑马鱼幼虫中的ClO-。
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| 关 键 词： | 荧光靶向探针 次氯酸盐 斑马鱼成像 香豆素 |
| 收稿时间： | 2022/2/11 0:00:00 |
| 修稿时间： | 2022/5/9 0:00:00 |
A Fas-Responsive Mitochondria-Targeting Fluorescent Probe Detecting Hypochlorite in Living Cells and Zebrafish |
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| ZHANG Chang-Li,ZHANG Jing-Jing,SHEN You,LU Jia-Cheng,HUANG Fang,XU Li.A Fas-Responsive Mitochondria-Targeting Fluorescent Probe Detecting Hypochlorite in Living Cells and Zebrafish[J].Chinese Journal of Inorganic Chemistry,2022,38(8):1623-1632. |
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| Authors: | ZHANG Chang-Li ZHANG Jing-Jing SHEN You LU Jia-Cheng HUANG Fang XU Li |
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| Institution: | Key Laboratory of Advanced Functional Materials of Nanjing, School of Environmental Science, Nanjing Xiaozhuang University, Nanjing 211171, China;College of Science, Nanjing Forestry University, Nanjing 210093, China |
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| Abstract: | Herein, a mitochondria-targeting fluorescent probe Cou-Py has been designed and synthesized for sensing hypochlorite (ClO-).Cou-Py exhibited very weak fluorescence due to the C=N isomerization of the oxime group in the excited state and recovers fluorescence within 5 s based on a ClO--triggered deoximation reaction. Additionally, Cou-Py showed high selectivity toward ClO- over other reactive oxygen species (ROS) and owned a low detection limit (6.87 nmol·L-1) for ClO-. Importantly, Cou-Py has been successfully employed for visualizing ClO- in the mitochondria of MCF-7 cells as well as zebrafish larvae. |
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| Keywords: | fluorescent targeting probe hypochlorite zebrafish imaging coumarin |
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