基于苯并噻唑Zn2+荧光探针及其细胞造影应用

设计合成基于苯并噻唑Zn²⁺荧光增强型探针BHP，在HEPES缓冲液中测其对Zn²⁺识别性能。实验结果表明，BHP对Zn²⁺有较高的选择性，对其他金属离子如Cd²⁺，Fe²⁺，Ni²⁺，Pb²⁺，Hg²⁺，Al³⁺，Mn²⁺，Ag⁺，Cu²⁺，Co²⁺，Na⁺，K⁺，Mg²⁺和Ca²⁺无明显荧光增强响应。BHP与Zn²⁺按1：1计量比配位，在生理条件下荧光强度不受pH值影响。在HeLa细胞中对Zn²⁺的造影表明BHP可用于生物体Zn²⁺检测。

Benzothiazole-based Zn2+-Specific Fluorescent Probe and Its Cell Imaging Application

A fluorescent probe 2-((2-(benzod]thiazol-2-yl)hydrazono)methyl)-5-(diethylamino) phenol (BHP) was designed and synthesized as a Zn²⁺ selective fluorescent chemosensor. It demonstrates Zn²⁺-specific emission enhancement, while many other ions such as Cd²⁺, Fe²⁺, Ni²⁺, Pb²⁺, Hg²⁺, Al³⁺, Mn²⁺, Ag⁺, Cu²⁺, Co²⁺, Na⁺, K⁺, Mg²⁺ and Ca²⁺, have no response. Notably, this chemosensor can distinguish Zn²⁺ from Cd²⁺. Its binding stoichiometry with Zn²⁺ is 1:1 ratio. Its pH-independent Zn²⁺-induced emission enhancement in physiological condition and cell permeability make it an effective intracellular Zn²⁺ imaging agent.