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| Ag+与Hg2+对G-四链体DNA结构的破坏及其对构筑DNA逻辑门的应用 | |
| 引用本文： | 朱莉娜,王海仙,孙润丰,李平,孔德明,李孝增.Ag+与Hg2+对G-四链体DNA结构的破坏及其对构筑DNA逻辑门的应用[J].无机化学学报,2013,29(10):2215-2224. |
| 作者姓名： | 朱莉娜 王海仙 孙润丰 李平 孔德明 李孝增 |
| 作者单位： | 1. 天津大学化学系,天津,300072 2. 青海民族学院外语系,西宁,810007 3. 南开大学教育部功能高分子材料重点实验室,天津,300071 |
| 基金项目： | 国家自然科学基金(No.20801040,21175072)资助项目 |
| 摘 要： | 本工作利用圆二色光谱研究了Ag+与Hg2+对4种代表性G-四链体DNA结构的破坏作用。结果表明Ag+可能通过与碱基G螯合从而破坏G-四链体结构;Hg2+能通过形成T-Hg2+-T碱基对,及其他方式破坏G-四链体结构。含巯基(-SH)的半胱氨酸与Ag+与Hg2+可以发生较强的配位作用,从而使被Ag+与Hg2+破坏后的G-四链体DNA结构得以回复。基于此,一个新颖的Ag+/Hg2+-半胱氨酸-DNA逻辑门得以构筑。 |
| 关 键 词： | G-四链体 DNA逻辑门 银离子 汞离子 半胱氨酸 圆二色光谱 |
| 收稿时间： | 3/1/2013 12:00:00 AM |
| 修稿时间： | 2013/4/19 0:00:00 |
Disruption of G-Quadruplex DNA by Ag+ and Hg2+ and Its Application to DNA Logic Gate |
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| ZHU Li-N,WANG Hai-Xian,SUN Run-Feng,LI Ping,KONG De-Ming and LI Xiao-Zeng.Disruption of G-Quadruplex DNA by Ag+ and Hg2+ and Its Application to DNA Logic Gate[J].Chinese Journal of Inorganic Chemistry,2013,29(10):2215-2224. | |
| Authors: | ZHU Li-N WANG Hai-Xian SUN Run-Feng LI Ping KONG De-Ming and LI Xiao-Zeng |
| Institution: | Department of Chemistry, Tianjin University, Tianjin 300072, China;Department of Chemistry, Tianjin University, Tianjin 300072, China;School of Foreign Languages, Qinghai University for Nationalities, Xining 810007, China;Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Nankai University, Tianjin 300071, China;Key Laboratory of Functional Polymer Materials (Nankai University), Ministry of Education, Nankai University, Tianjin 300071, China;Department of Chemistry, Tianjin University, Tianjin 300072, China |
| Abstract: | The G-quadruplex-disrupting abilities of Ag and Hg2 ions were investigated by utilizing the metal ions-mediated circular dichroism (CD) spectrum changes of four representative G-quadruplexes. The results show that Ag ion can disrupt G-quadruplexes by chelating to Gbases and thus can be used as a general G-quadruplex-disrupting agent. The results also suggest that Hg2 ion may disrupt G-quadruplexes by formation of T-Hg2 -Tbase pairs as well as other species. The G-quadruplexes disrupted by Ag and Hg2 can be recovered by cysteine (Cys), a thiol-containing amino acid, due to the tight binding interactions between Cys and the metal ions. Thus, the system by using Ag (or Hg2 ) and Cys as two inputs and CDsignal as output can behave as a DNA IMPLICATIONlogic gate. |
| Keywords: | G-quadruplex DNA logic gate Ag Hg2 cysteine circular dichroism |
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