| Detection of Hepatitis B Virus DNA by Duplex Scorpion Primer-based PCR Assay |
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| 作者姓名： | 孔德明 沈含照 宓怀风 |
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| 作者单位： | [1]StateKeyLaboratoryofFunctionalPolymerMaterialsforAdsorptionandSeparation,ChemicalSchool,NankaiUniversity,Tianjin300071,China [2]ChemicalSchool,NankaiUniversity,Tianjin300071,China |
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| 基金项目： | the National Natural Science Foundation of China (No. 20075012) and the Outstanding Scholar Program of Nankai University. |
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| 摘 要： | The application of a new fluorogenic probe-based PCR assay (PCR duplex scorpion primer assay) to the detection of Hepatitis B virus (HBV) DNA in human sera was described. Duplex scorpion primer is a modified variant of duplex Amplifluor, and the incorporation of a PCR stopper between probe and primer sequences improve the detection specificity and sensitivity. Combined with PCR amplification, this probe can give unambiguous positive results for the reactions initiated with more than 20 HBV molecules. In addition, the particular unimolecular probing mechanism of this probe makes the use of short target-specific probe sequence possible, which will render this probe applicable in some specific systems.
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| 关 键 词： | 乙型肝炎病毒 DNA 提取 PCR 荧光探针 |
Detection of Hepatitis B Virus DNA by Duplex Scorpion Primer-based PCR Assay |
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| KONG,De-Ming,a,b SHEN,Han-XibMI,Huai-Fengaa State Key Laboratory of Functional Polymer Materials for Adsorption and Separation,Chemical School,Nankai University,Tianjin ,China b Chemical School,Nankai University,Tianjin ,China.Detection of Hepatitis B Virus DNA by Duplex Scorpion Primer-based PCR Assay[J].Chinese Journal of Chemistry,2004,22(9):903-907. |
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| Authors: | KONG De-Ming a b SHEN Han-XibMI Huai-Fengaa State Key Laboratory of Functional Polymer Materials for Adsorption and Separation Chemical School Nankai University Tianjin China b Chemical School Nankai University Tianjin China |
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| Institution: | KONG,De-Ming*,a,b SHEN,Han-XibMI,Huai-Fengaa State Key Laboratory of Functional Polymer Materials for Adsorption and Separation,Chemical School,Nankai University,Tianjin 300071,China b Chemical School,Nankai University,Tianjin 300071,China |
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| Abstract: | The application of a new fluorogenic probe-based PCR assay (PCR duplex scorpion primer assay) to the detec-tion of Hepatitis B virus (HBV) DNA in human sera was described. Duplex scorpion primer is a modified variant of duplex Amplifluor, and the incorporation of a PCR stopper between probe and primer sequences improve the detec-tion specificity and sensitivity. Combined with PCR amplification, this probe can give unambiguous positive results for the reactions initiated with more than 20 HBV molecules. In addition, the particular unimolecular probing mechanism of this probe makes the use of short target-specific probe sequence possible, which will render this probe applicable in some specific systems. |
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| Keywords: | duplex scorpion primer hepatitis B virus (HBV) DNA fluorogenic probe |
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