铂纳米颗粒修饰电极对大肠杆菌的电化学快速检测

本文采用了电化学沉积法制备了铂纳米颗粒化学修饰电极（PtNP/GCE），并将它应用于大肠杆菌的检测。原理是基于检测大肠杆菌溶液中酶与底物的反应产物，对氨基酚，实现了对大肠杆菌的快速检测。采用了铂纳米颗粒修饰电极，并对检测系统进行优化，提高大肠杆菌的检测灵敏度。大肠杆菌浓度在50—1.0×10⁵cfu/ml与响应电流成良好的线性关系，最低检测限为20 cfu/ml，检测时间在4个小时以内。与传统方法相比，该电化学方法能很好地满足食品安全、环境监控和临床医学等领域中快速检测的要求。

Platinum Nanoparticles Modified Electrode for Rapid Electrochemical Detection of Escherichia coli

A platinum nanoparticles chemically modified electrode (PtNP/GCE) was fabricated by electro‐deposition and used to detection of Escherichia coli (E. coli). The detection principle was based on determination of p‐aminophenol that was produced by an enzymatic reaction in E. coli solution. The sensitivity of the detection was improved by the platinum nanoparticle modified electrode and optimization of the detection system. The current responses were proportional to the density of E. coli ranging from 50 to 1.0×10⁵ cfu/mL, with detection limit of 20 cfu/mL, and the detection time was less than 4 h. Compared with conventional methods, the electrochemical technology described here could be suitable for rapid detection of E. coli in the fields of food industry, environmental monitoring and clinic biomedicine.