| 光谱法研究噻菁染料与血清蛋白的相互作用 |
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| 引用本文： | 张亚周,杨千帆,杜鸿雁,唐亚林,徐广智,闫文鹏.光谱法研究噻菁染料与血清蛋白的相互作用[J].中国化学,2008,26(2):397-401. |
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| 作者姓名： | 张亚周 杨千帆 杜鸿雁 唐亚林 徐广智 闫文鹏 |
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| 作者单位： | 北京分子科学国家实验室, 中国科学院化学研究所分子动态稳态国家重点实验室, 中国 北京 100080 中国科学院研究生院, 中国, 北京 100080 中国科学院理化技术研究所, 中国, 北京 100080 |
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| 摘 要： | 本文通过吸收和荧光光谱法研究了一种噻菁染料与人血清蛋白及牛血清蛋白的相互作用。吸收光谱数据表明，与血清蛋白结合后，噻菁染料单体的吸收峰发生红移，同时强度也有很大变化；还通过吸收光谱计算确定了噻菁染料与血清蛋白的结合位点数（ n ）。与人血清蛋白或牛血清蛋白结合后，噻菁染料的荧光量子产率增加。分析噻菁染料的荧光强度随溶液中血清蛋白浓度的变化得到了二者反应的表观结合常数（ K a）和自由能变化（ ΔG ）。根据表观结合常数（ K a）可以判断，人血清蛋白比牛血清蛋白与噻菁染料的结合更强。
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| 关 键 词： | 人血清蛋白 牛血清蛋白 荧光探针 噻菁染料 |
| 收稿时间： | 2007-5-9 |
| 修稿时间： | 2007-9-19 |
Spectroscopic Investigation on the Interaction of a Cyanine Dye with Serum Albumins |
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| Ya‐Zhou ZHANG,Qian‐Fan YANG,Hong‐Yan DU,Ya‐Lin TANG,Guang‐Zhi XU,Wen‐Peng YAN.Spectroscopic Investigation on the Interaction of a Cyanine Dye with Serum Albumins[J].Chinese Journal of Chemistry,2008,26(2):397-401. |
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| Authors: | Ya‐Zhou ZHANG Qian‐Fan YANG Hong‐Yan DU Ya‐Lin TANG Guang‐Zhi XU Wen‐Peng YAN |
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| Institution: | 1. Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China;2. Graduate University of Chinese Academy of Sciences, Beijing 100080, China;3. Tel.: 027‐87543232;4. Fax: 086‐027‐87543632;5. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | The interactions of a cyanine dye with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by using absorption and fluorescence spectra. Absorption spectral studies show that binding to the serum albumins leads to a bathochromic shift of the monomer band together with a notable intensity change. Furthermore, the number of binding sites (n) was identified by the absorption spectra. There is a constant enhancement of fluorescence quantum yield when the cyanine dye complexes with HSA or BSA. The apparent binding constant (Ka) and the free energy changes (ΔG) were obtained by analysis of fluorescence data of the cyanine dye in the absence and presence of HSA and BSA. Compared to BSA, HSA associates with the dye in a stronger way. |
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| Keywords: | human serum albumin bovine serum albumin fluorescent probe cyanine dye |
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