中性红掺杂SiO2纳米粒子修饰的酶阵列传感器同时测定葡萄糖、乳酸和L-谷氨酸的研究

本文以中性红为核，二氧化硅为壳，利用反相微乳液技术，通过正硅酸四乙酯的水解制备了掺杂有中性红的二氧化硅纳米粒子，并用TEM技术进行了表征。核中性红能够催化测定葡萄糖，乳酸和L-谷氨酸的反应，而壳二氧化硅不仅克服了电活性物质中性红易流失的缺点，且具有高的生物亲和性。分别与葡萄糖氧化酶、乳酸氧化酶以及L-谷氨酸氧化酶混合后，修饰在碳阵列电极表面。最后在该酶阵列电极表面滴加一层Nafion, 防止电活性物质抗坏血酸、尿酸等的干扰。该酶阵列传感器与流动注射分析技术(FIA)相结合，可应用于同时检测大鼠血样中的葡萄糖，乳酸和L-谷氨酸浓度。该方法无需通过传统的色谱柱的分离，大大简化了实验条件，为这一领域的研究提供了有效的分析方法。

Simultaneous Monitoring of Glucose,Lactate and L‐Glutamate in Rat Blood by a Flow‐injection Enzyme Electrode Array System

Rapid measurement of glucose, lactate and L‐glutamate level in blood is important for studying the balance of energy in body. The flow‐injection analysis (FIA) system with enzyme electrode array was based on neutral red‐doped silica (NRDS) nanoparticles as electrocatalyst. These uniform NRDS nanoparticles (about 50±3 nm) were prepared by a water‐in‐oil (W/O) microemulsion method, and characterized by TEM technique. The doped in‐side neutral red maintained its high electron‐activity, while the outside nano silica surface prevented neutral red from leaching out into the aqueous solutions and showed high biocompability. These nanoparticles were then mixed with the glucose oxidase (GOD), lactate oxidase (LOD) or L‐glutamate oxidase (L‐GLOD), and immobilized on a three carbon‐disk electrode (CE) array, respectively. A thin Nafion film was coated on the enzyme layer to prevent interference such as ascorbic acid and uric acid in the blood. The proposed flow‐injection analysis with NRDS‐enzyme electrode array method enables simultaneously monitoring various levels of glucose, lactate and L‐glutamate in blood