| Microcalorimetric Study of the Biological Effects of Zn^＋ on Bacillus thuringiensis Growth |
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| 引用本文： | 姚俊,刘义,等.Microcalorimetric Study of the Biological Effects of Zn^＋ on Bacillus thuringiensis Growth[J].中国化学,2002,20(8):746-746. |
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| 作者姓名： | 姚俊 刘义 |
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| 作者单位： | [1]CollegeofLifeScienceandChemistry,JishouUniversity,Jishou,Hunan416000,China [2]DepartmentofChemistry,CollegeofChemistryandMolecularScience,Wuhan,University,Wuhan,Hubei430072,China |
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| 摘 要： | A microcalorimetric technique was used to investigate the influence of Zn^2 on the growth metabolism of Bacillus thuringiensis .LKB-2277 Bioactivity Monitor was employed to obtain the power-time curves,from which the maximum peak-heat output power(Pmax) in the log phase,the growth rate constants(k), the inhibitory ratios(I) ,the generational time(tG) and the total heat effect (Qtotal) in 23 h for the growth metabolism of Bacillus thuringiensis at 28℃ can be evaluated,The results indicate that the concentration of Zn^2 affects its growth obviously,Low concentration (0-50μg/mL) of Zn^2 promotes the growth of Bacillus thuringiensis while high concentration (50-500μg/mL) of Zn^2 inhibits its growth .When the concentration reached up to 600μg/mL,it can not grow at all.
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| 关 键 词： | 杆菌 细胞生长 锌（Ⅱ） 浓度 生物活性 微量热法 |
Microcalorimetric Study of the Biological Effects of Zn2+ on Bacillus thuringiensis Growth |
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| Jun Yao,Yi Liu,Zhen‐Ting Gao,Peng Liu,Ming Sun,Xue Zou,Song‐Sheng Qu,Zi‐Niu Yu.Microcalorimetric Study of the Biological Effects of Zn2+ on Bacillus thuringiensis Growth[J].Chinese Journal of Chemistry,2002,20(8):746-746. |
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| Authors: | Jun Yao Yi Liu Zhen‐Ting Gao Peng Liu Ming Sun Xue Zou Song‐Sheng Qu Zi‐Niu Yu |
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| Abstract: | A microcalorimetric technique was used to investigate the influence of Zn2+ on the growth metabolism of Bacillus thuringiensis. LKB‐2277 Bioactivity Monitor was employed to obtain the power‐time curves, from which the maximum peak‐heat output power (Pmax) in the log phase, the growth rate constants (k), the inhibitory ratios (I), the generational time (tG) and the total heat effect (Qtotal) in 23 h for the growth metabolism of Bacillus thuringiensis at 28 °C can be evaluated. The results indicate that the concentration of Zn2+ affects its growth obviously. Low concentration (0–50 μg/mL) of Zn2+ promotes the growth of Bacillus thuringiensis while high concentration (50–500 μg/mL) of Zn2+ inhibits its growth. When the concentration reached up to 600 μg/mL, it can not grow at all. |
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| Keywords: | Bacillus thuringiensis Zn2+ growth metabolism microcalorimetry |
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