Impact of Magnesium(II) on Beryllium Fluorides in Solutions Studied by 19F NMR Spectroscopy |
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| Authors: | Yixiang Liu Xian Mao Maili Liu Ling Jiang |
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| Institution: | 1. Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Center for Magnetic Resonance, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, Hubei 430071, China;2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China;3. Department of Pharmacology, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA |
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| Abstract: | Beryllium fluorides are widely used in protein phosphorylation studies to get stable transition state analogs or near attack conformers, which has attracted much attention. BeF3? is one of the optimal phosphoryl (PO3?) analogs for its identical geometry and charge, and Mg2+ naturally participates in the phosphoryl binding in biological systems. In solutions, BeF3? coexists with other beryllium fluorides (BeF42?, BeF2 and BeF+) and magnesium fluorides, and there are equilibriums between these species. In this article, 19F NMR spectroscopy was applied to the investigation of the impact of magnesium(II) on beryllium fluorides. It has been found that when Mg2+ was introduced into the solutions, the chemical shifts, the intensities and the line widths of 19F signals of various beryllium fluoride complexes were changed. After ionic strength correction, these effects were remarkable only for BeF42? and BeF3?, especially BeF42?, when the concentration of the fluoride ion is relatively low. Mechanism of the effects is proposed which involves ion pair formation between Mg2+ and beryllium fluorides. |
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| Keywords: | beryllium fluorides magnesium(II) 19F NMR ion pair protein phosphorylation |
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