| A New Nanogold-Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG |
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| 作者姓名： | 蒋治良 李燕 孙双娇 谌斌 |
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| 作者单位： | [1]School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China [2]Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （Nos. 20667001, 20365001）, Natural Science Foundation of Guangxi （No. 2060213） and the Foundation of New Century Ten-Hundred-Thousand Talents of Guangxi. |
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| 摘 要： | A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti-human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol （PEG）, the immune reaction between gold-labeled goat anti-human IgG and IgG took place, and the resonance scattering intensity at 580 nm （I580nm） was enhanced greatly. The enhanced intensity AIRS is pro- portional to the IgG concentration from 1.3 to 1.5 X 10^3 ng.mL^-1, with a detection limit of 0.78 ng.mL ^-1. This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results.
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| 关 键 词： | IgG 共振射散 光谱 化学 |
| 修稿时间： | 2006-11-09 |
A New Nanogold-Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG |
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| JIANG Zhi-Liang, LI Yan,SUN Shuang-Jiao, CHEN Binga.A New Nanogold-Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG[J].Chinese Journal of Chemistry,2007,25(9):1282-1287. |
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| Authors: | JIANG Zhi-Liang LI Yan SUN Shuang-Jiao CHEN Binga |
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| Institution: | 1. School of Environment and Resource, Guangxi Normal University, Guilin, Guangxi 541004, China ;2. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin, Guangxi 541004, China |
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| Abstract: | A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti‐human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold‐labeled goat anti‐human IgG and IgG took place, and the resonance scattering intensity at 580 nm (I580 nm) was enhanced greatly. The enhanced intensity ΔIRS is proportional to the IgG concentration from 1.3 to 1.5×103 ng·mL?1, with a detection limit of 0.78 ng·mL?1. This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results. |
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| Keywords: | IgG resonance scattering nanogold-labeled immunoresonance scattering probe |
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