| Spectral Studies on the Interaction between Mercuric Ion and ApoCopC |
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| 作者姓名： | 郑晓艳 庞尔国 李慧卿 赵亚琴 杨斌盛 |
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| 作者单位： | Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, Shanxi 030006, China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China （No. 20371031） and the Natural Science Foundation of Shanxi Province （No. 20031017）. |
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| 摘 要： | The interaction between mercuric ion and apoCopC in the absence or presence of cupric ion was investigated through difference UV spectra in Hepes buffer （10 mmol·L^-1） at pH 7.4. The results suggest that mercuric ion can bind to C- and N-terminal binding sites of apoCopC, and the conditional binding constants were calculated to be kN=（6.79± 1.12）× 10^6 mol^-1·L and kc=（3.06±0.05）× 10^5 mol^-1·L. Using urea as a chemical agent, the conformational stabilities of apoCopC and HgN^2＋ -CopC-Hgc^2＋ were monitored by fluorescence spectrum in Hepes buffer （50 mmol·L^-1） at pH 7.4. The free energy of stabilization is （14.69±0.85） and （16.66±0.55） kJ.mol^-1, respectively. HgN^2＋ -CopC-Hgc^2＋ is more stable than apoCopC.
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| 关 键 词： | 汞离子 ApoCopC 蛋白质 相互作用 铜 尿素 光谱 重金属污染 |
| 修稿时间： | 2006-05-222007-01-24 |
Spectral Studies on the Interaction between Mercuric Ion and ApoCopC |
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| ZHENG, Xiao-Yan PANG, Er-Guo Hui-Qing ZHAO, Ya-Qin YANG, Bin-Sheng.Spectral Studies on the Interaction between Mercuric Ion and ApoCopC[J].Chinese Journal of Chemistry,2007,25(5):630-634. |
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| Authors: | ZHENG Xiao-Yan PANG Er-Guo Hui-Qing ZHAO Ya-Qin YANG Bin-Sheng |
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| Institution: | Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, Shanxi 030006, China |
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| Abstract: | The interaction between mercuric ion and apoCopC in the absence or presence of cupric ion was investigated through difference UV spectra in Hepes buffer (10 mmol·L?1) at pH 7.4. The results suggest that mercuric ion can bind to C‐ and N‐terminal binding sites of apoCopC, and the conditional binding constants were calculated to bekN=(6.79±1.12)×106 mol?1·L and kC=(3.06±0.05)×105 mol?1·L. Using urea as a chemical agent, the conformational stabilities of apoCopC and Hg2+N‐CopC‐Hg2+C were monitored by fluorescence spectrum in Hepes buffer (50 mmol·L?1) at pH 7.4. The free energy of stabilization is (14.69±0.85) and (16.66±0.55) kJ·mol?1, respectively. Hg2+N‐CopC‐Hg2+C is more stable than apoCopC. |
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| Keywords: | apoCopC copper mercuric urea spectra |
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