| Influences of Triton X-100 on Hemoglobin Behaviors in Hemoglobin/Acyclovir/Triton X-100/H2O System |
| |
| 作者姓名： | 刘天晴 郭荣 |
| |
| 作者单位： | [1]School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jinagsu 25002, China [2]School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jinagsu 210093, China |
| |
| 基金项目： | Project supported by the National Natural Science Foundation of China （Nos. 20573091, 20233010）. |
| |
| 摘 要： | The influences of Triton X-100 on hemoglobin （Hb） behaviors were studied by the methods of UV-Vis spectrum, fluorescence spectrum, HPLC, conductivity, zeta potential and negative-staining transmission electron microscope in Hb/acyclovir/Triton X-100/H2O system. With the increase of Triton X-100 concentration in the system, the percentage of the free acyclovir increased from 58%--63% to 90%--94%. The static quenching constant and the association number of acyclovir to Hb decreased. The fluorescence spectrum, conductivity, zeta potential, fluorescence polarization and negative-staining morphology of Hb tended to recover to those of the original state of Hb in the same concentration of Hb. The interaction between Triton X-100 and Hb is stronger than that between acyclovir and Hb. Most Triton-X-100 was associated with Hb at low Triton X-100 concentration. But the interaction of Triton X-100 with Hb was apparently dominant in high Triton X-100 concentration. The Hb structure was unfolded and finally denatured.
|
| 关 键 词： | 血红蛋白 阿昔洛韦 Triton X-100 特性 药物输送 非离子型表面活性剂 |
| 修稿时间： | 2005-12-292006-12-22 |
Influences of Triton X-100 on Hemoglobin Behaviors in Hemoglobin/Acyclovir/Triton X-100/H2O System |
| |
| LIU Tian-Qing, GUO Rong.Influences of Triton X-100 on Hemoglobin Behaviors in Hemoglobin/Acyclovir/Triton X-100/H2O System[J].Chinese Journal of Chemistry,2007,25(4):490-497. |
| |
| Authors: | LIU Tian-Qing GUO Rong |
| |
| Institution: | 1. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jinagsu 25002, China; 2.School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jinagsu 210093, China |
| |
| Abstract: | The influences of Triton X‐100 on hemoglobin (Hb) behaviors were studied by the methods of UV‐Vis spectrum, fluorescence spectrum, HPLC, conductivity, zeta potential and negative‐staining transmission electron microscope in Hb/acyclovir/Triton X‐100/H2O system. With the increase of Triton X‐100 concentration in the system, the percentage of the free acyclovir increased from 58%–63% to 90%–94%. The static quenching constant and the association number of acyclovir to Hb decreased. The fluorescence spectrum, conductivity, zeta potential, fluorescence polarization and negative‐staining morphology of Hb tended to recover to those of the original state of Hb in the same concentration of Hb. The interaction between Triton X‐100 and Hb is stronger than that between acyclovir and Hb. Most Triton‐X‐100 was associated with Hb at low Triton X‐100 concentration. But the interaction of Triton X‐100 with Hb was apparently dominant in high Triton X‐100 concentration. The Hb structure was unfolded and finally denatured. |
| |
| Keywords: | influence behavior hemoglobin Triton X-100 acyclovir |
| 本文献已被 维普 等数据库收录！ |
|
