| GIF S-亚硝基化的一些见解 |
| |
| 引用本文： | 滕昕辰,郑起,蔡斌,倪峰云,谢毅,孙红哲,张明杰,黄仲贤.GIF S-亚硝基化的一些见解[J].中国化学,2005,23(11):1545-1551. |
| |
| 作者姓名： | 滕昕辰 郑起 蔡斌 倪峰云 谢毅 孙红哲 张明杰 黄仲贤 |
| |
| 作者单位： | [1]Department of Chemistry [2]Institute of Genetics, Life Science School, Fudan University, Shanghai 200433, China [3]Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, China [4]Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China |
| |
| 基金项目： | Project supported by the National Natural Science-Foundation of China （No. 30229001）. |
| |
| 摘 要： | Neural growth inhibitory factor （GIF）, a member of metallothionein family （metallothionein-3, MT3）, was well known by its distinct neural growth inhibitory activity, which is not shown by other MT isoforms. However, till now, people still did not know clearly how GIF exerts its biological functions. Since it has been reported that GIF might serve as NO scavenger and was related to the release of zinc, our study was focused on the interaction of GIF and NO. By studying the reactions of human GIF and human MTlg with SNOC-a type of NO donor, it was found that GIF was more reactive than MT-lg toward SNOC. In order to further figure out if the high reactivity of GIF in this reaction resulted from the acid-base catalysis, several mutants were constructed： E23K, E41G/E43A, E23K/E41G/E43A. By studying their basic properties and the reactions toward SNOC, it was found that the S-nitrosylation of GIF was not only related to the acid-base catalysis, but also to the accessibility of metal-thiolate clusters.
|
| 关 键 词： | 人体 生长抑制因素 催化剂 疾病 GIF |
| 收稿时间： | 2005-03-02 |
| 修稿时间： | 2005-03-022005-07-05 |
An Insight of S-Nitrosylation of Human GIF |
| |
| TENG,;Xin-Chen;ZHENG,;Qi;CAI,;Bin;NI,;Feng-Yun;XlE,;yi;SUN,;Hong-Zhe;ZHANG,;Ming-Jie;HUANG,;Zhong-Xian.An Insight of S-Nitrosylation of Human GIF[J].Chinese Journal of Chemistry,2005,23(11):1545-1551. |
| |
| Authors: | TENG ;Xin-Chen;ZHENG ;Qi;CAI ;Bin;NI ;Feng-Yun;XlE ;yi;SUN ;Hong-Zhe;ZHANG ;Ming-Jie;HUANG ;Zhong-Xian |
| |
| Abstract: | Neural growth inhibitory factor (GIF), a member of metallothionein family (metallothionein‐3, MT3), was well known by its distinct neural growth inhibitory activity, which is not shown by other MT isoforms. However, till now, people still did not know clearly how GIF exerts its biological functions. Since it has been reported that GIF might serve as NO scavenger and was related to the release of zinc, our study was focused on the interaction of GIF and NO. By studying the reactions of human GIF and human MT1g with SNOC–a type of NO donor, it was found that GIF was more reactive than MT‐1g toward SNOC. In order to further figure out if the high reactivity of GIF in this reaction resulted from the acid‐base catalysis, several mutants were constructed: E23K, E41G/E43A, E23K/E41G/E43A. By studying their basic properties and the reactions toward SNOC, it was found that the S‐nitrosylation of GIF was not only related to the acid‐base catalysis, but also to the accessibility of metal‐thiolate clusters. |
| |
| Keywords: | growth inhibitory factor metallothionein S-nitrosylation acid-base catalysis |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《中国化学》浏览原始摘要信息 |
| 点击此处可从《中国化学》下载免费的PDF全文 |
|
