A Novel MALDI Matrix for Analyzing Peptides and Proteins: Paraffin Wax Immobilized Matrix |
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| Authors: | WEI Yuanlong MEI Yuan XU Zhe WANG Cuihong GUO Yinlong DU Yiping ZHANG Weibing |
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| Institution: | 1. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China;2. Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China;3. Fax: 0086‐21‐64166128;4. Fax: 0086‐21‐64252947 |
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| Abstract: | A new kind of MALDI matrix, termed paraffin wax immobilized matrix, was used to study peptide mixtures and proteins. During the preparation process, the paraffin wax was heated and coated on the stainless‐steel target plate, and then 2,5‐dihydrobenzoic acid (DHB) was deposited on the paraffin layer and stainless‐steel target plate to obtain different kinds of matrix spots. The morphology of matrices on different supports and peptide‐matrix co‐crystallization were observed by a high resolution digital‐video microscopy system. Peptide mixtures and bovine serum albumin (BSA) digests were used to investigate the performance of the immobilized matrices on the paraffin target. The MALDI‐FTMS analysis results also showed that the detection sensitivity of matrices immobilized in the paraffin sample support was better than that on other sample supports. |
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| Keywords: | MALDI‐FTMS paraffin wax immobilized matrix morphology protein 2 5‐dihydrobenzoic acid (DHB) |
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