| Capillary Zone Electrophoresis with Amperometric Detection for Composition Analysis of Laminarin |
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| 作者姓名： | 王清江 丁飞 李辉 何品刚 方禹之 |
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| 作者单位： | Department of Chemistry，East China Normal University，Shanghai 200062，China，Department of Chemistry，East China Normal University，Shanghai 200062，China，School of Chemistry and Chemical Engineering，Shanghai Jiaotong University，Shanghai 200240，China，Department of Chemistry，East China Normal University，Shanghai 200062，China，Department of Chemistry，East China Normal University，Shanghai 200062，China |
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| 基金项目： | theFoundationofStateKeyLaboratoryofElectroanalyticalChemistry ,ChangchunInstituteofAppliedChemistry ,ChineseAcademyofSciences |
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| 摘 要： | IntroductionInrecentdecades ,manykindsofplantpolysaccha rideshavebeenextensivelystudiedfortheirpotentialvalueinimmunology ,especiallyincancertherapeusis .Laminar in ,onekindofheteropolysaccharidesisolatedfromLami nariajaponica ,wasfoundtobeoneoftheinhibitorsofba sicfabriccell generationfactor (bFGF)anddepresstheformationoftubestructureofendothelialcells ,whichfur therdepresstheactivationofrats’cancercells .1Itwasal sofoundthatlaminarinsulfatecouldinhibitextracellularmatrix (ECM)degradation…
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| 关 键 词： | 毛细管电泳 电流测定 昆布多糖 成分分析 多糖化合物 抗癌药物 药物分析 |
Capillary Zone Electrophoresis with Amperometric Detection for Composition Analysis of Laminarin |
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| WANG,Qing-Jiang a DING,Fei a LI,Hui b HE,Pin-Gang a FANG,Yu-Zhi,a a.Capillary Zone Electrophoresis with Amperometric Detection for Composition Analysis of Laminarin[J].Chinese Journal of Chemistry,2003,21(9):1198-1202. |
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| Authors: | WANG Qing-Jiang a DING Fei a LI Hui b HE Pin-Gang a FANG Yu-Zhi a a |
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| Institution: | WANG,Qing-Jiang a DING,Fei a LI,Hui b HE,Pin-Gang a FANG,Yu-Zhi,a a Department of Chemistry,East China Normal University,Shanghai 200062,China b School of Chemistry and Chemical Engineering,Shanghai Jiaotong University,Shanghai 200240,China |
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| Abstract: | The composition of laminarin was firstly determined by analyzing its hydrolysis monosaccharides with capillary zone electrophoresis‐amperometric detection (CZE‐AD). Under the selected optimum conditions, fucose, galactose, glucose, mannose and xylose, which are hydrolysis products of laminarin, could be perfectly separated within 20 min and showed significant current responses at copper electrodes. The linear ranges of fucose, galactose and glucose were from 1.0 ± 10–6 to 2.0 ± 10–4 mol ± L?1, those of mannose and xylose were from 5.0 ± 10–6 to 2.0 ± 10–4 mol ± L?1, and their detection limits were at 10–7mol ± L?1 level (S/N = 3). The molar ratio of fucose, galactose, glucose, mannose and xylose in laminarin was 10.5: 2.8:1.0:7.3: 3.4 and the purity of this polysaccharide leached by the introduced leaching method was 95.7%. Compared to usual UV‐vis and other spectrometric methods, analyzing polysaccharide by this method has some merits of quickness, low‐volume sampling, simple instrumentation, high sensitivity and high reproducibility. |
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| Keywords: | laminarin capillary zone electrophoresis amperometric detection |
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