铁氰化钾高灵敏共振瑞利散射法测定青霉素类抗生素

在稀HCl介质中，K3Fe(CN)6]与阿莫西林(AMO)、氨苄西林(AMP)、氯唑西林钠(CLO)、羧苄西林钠(CAR)和青霉素钠(BEN)等抗生素药物在加热条件下反应生成结合产物，会导致溶液的共振瑞利散射(RRS)强度急剧增强，并产生新的RRS光谱,5种反应产物的最大散射峰均位于330 nm附近。在一定的浓度范围内，不同的反应体系散射强度(∆I)与药物浓度成正比，反应具有很高的灵敏度，K3Fe(CN)6]对5种药物的检出限分别在4.61至5.62 ng·mL-1之间。本文研究了RRS的光谱特征和适当的反应条件，并讨论了反应机理和散射增强的原因，还考察了共存物质的影响,表明方法具有较好的选择性，可用于胶囊、片剂和人血清及尿液中青霉素类药物的测定。

A Highly Sensitive Resonance Rayleigh Scattering Method for the Determination of Penicillin Antibiotics with Potassium Ferricyanide

Heated in a boiling water bath, penicillin antibiotics such as amoxicillin, ampicillin, sodium cloxacillin, sodium carbenicillin and sodium benzylpenicillin could react with K₃Fe(CN)₆] to form combined products in a dilute HCl medium. As a result, resonance Rayleigh scattering (RRS) intensity was enhanced greatly and new RRS spectra appeared. The maximum scattering wavelengths of the five combined products are all located at 330 nm. The scattered intensity increments (ΔI) of the combined products are directly proportional to the concentrations of the antibiotics in a certain range. The methods exhibit high sensitivity, and the detection limits for the five penicillin antibiotics are between 4.61 and 5.62 ng·mL^?1. The spectral characteristics of RRS and the optimum reaction conditions were investigated. The mechanism of reaction and the reasons for the enhancement of resonance light scattering were discussed. The effects of coexisting substances have been examined, and the results indicated that the method had a good selectivity. It can be applied to the determination of penicillin antibiotics in capsule, tablet, human serum and urine samples.