In vitro and in vivo evaluation of chitosan-alginate/gentamicin wound dressing nanofibrous with high antibacterial performance |
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| Institution: | 1. School of Chemistry and Environment Science, Guangdong Ocean University, Zhanjiang 524088, PR China;2. Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524001, PR China;3. School of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang 524088, PR China;4. School of Nursing, Zhengzhou University, Zhengzhou, Henan Province 450001, PR China;1. Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, United States;2. Chongqing Academy of Animal Sciences and Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, China;3. Department of Surgery-General Surgery, University of Nebraska Medical Center, Omaha, NE, United States;4. Department of Orthopaedic Surgery and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, United States;5. Department of Orthopaedic Surgery, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV, United States;1. National University of Science and Technology “MISiS”, Leninsky pr. 4, 119049 Moscow, Russia;2. CEITEC-Central European Institute of Technology, Brno University of Technology, Technická 3058/10, 61600 Brno, Czech Republic;3. Institute of Physical Engineering, Brno University of Technology, Technicka 2896/2, 616 69 Brno, Czech Republic;4. State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region 142279, Russia;5. N.N. Blokhin Russian Cancer Research Center, Kashirskoe shosse 24, Moscow 115478, Russia;6. RG Plasma Technologies, CEITEC – Central European Institute of Technology, Masaryk University, Purkyňova 123, Brno 61200, Czech Republic;7. Department of Physical Electronics, Faculty of Science, Masaryk University, Kotlářská 2, Brno 61137, Czech Republic |
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| Abstract: | Wound dressings based on nanofiber polymer scaffolds with good antimicrobial performance and skin reconstruction ability are promising options to thwart wound infection and accelerate wound healing. This paper reports on the synthesis via electrospinning of chitosan-alginate (CS-Alg) nanofiber dressings with various amounts of gentamicin (Gn; 0–10 wt%) as a drug delivery system. Smooth and continuous nanofibers with no obvious beads were created, with increases in the amount of Gn resulting in reduced fiber diameter. Antimicrobial tests showed the Gn-loaded nanofibers had good antibacterial performance as indicated by the inhibition of bacterial growth. CS-Alg nanofibers loaded with higher Gn concentrations exhibited greater antibacterial performance than those with lower Gn concentrations. In vitro cell culture studies demonstrated that CS-Alg wound dressings with 1–3% Gn improved L929 cell attachment and proliferation more than wound dressings with higher Gn concentrations. In vivo experiments revealed that Cs-Alg nanofibers loaded with 3% Gn significantly enhanced skin regeneration in a Balb/C mice model by stimulating the formation of a thicker dermis, increasing collagen deposition, and increasing the formation of new blood vessels and hair follicles. Collectively, Gn-loaded CS-Alg wound dressings can be considered a good candidate for drug delivery systems and skin regeneration applications. |
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| Keywords: | Chitosan-alginate Electrospinning Wound dressings Antibacterial activity Biompatibility |
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