对氯苯甲酸构筑的铜(Ⅱ)配合物的合成、HSA结合及细胞毒性

本文合成了配合物Cu(pcba)₂·(phen)(H₂O)] (pcba =对氯苯甲酸,phen = 1,10-邻菲罗啉),该配合物属于三斜晶系,P1空间群,晶胞参数为a=0.790 98(2) nm,b=1.072 40(4) nm,c=1.487 19(6) nm,α=100.613(3)°,β=95.239(3)°,γ=108.334(3)°,Z=2,D_c=1.638 g·cm^-3,F(000)=582,最终结构残差因子R₁=0.035 9,wR₂=0.089 1。采用紫外及荧光研究了配合物和人血清蛋白(HSA)的相互作用方式。结果表明,配合物静态猝灭HSA荧光,可求得配合物与HSA的猝灭常数K_sv=2.35×10⁵ L·mol^-1,猝灭速率常数K_q=2.35×10¹³ L·mol^-1·s^-1,结合常数为K_a=2.14×10¹³ L·mol^-1,结合位点n=2.37。同时,研究了配合物对胃癌细胞A549、宫颈癌细胞Hela和肝癌细胞HepG2的抗增殖能力。

Synthesis,HSA Binding,and Cytotoxic Activity of Copper(Ⅱ) Complex Constructed by P-Chlorobenzoic Acid

In this paper,a copper(Ⅱ)complexCu(pcba)₂·(phen)(H₂ O)](pcba=p-chlorbenzoic acid,phen=1,10-phenanthroline)was synthesized.The structure of the complex belongs to triclinic,P1 space group,with a=0.79098(2)nm,b=1.07240(4)nm,c=1.48719(6)nm,α=100.613(3)°,β=95.239(3)°,γ=108.334(3)°,Z=2,D c=1.638 g·cm^-3,F(000)=582 unit cell parameters.The final R₁=0.0359,wR₂=0.0891.The interaction between HSA(human serum albumin)and the complex was evaluated by UV-Vis and fluorescence spectroscopy.The complex quenched the intrinsic fluorescence of HSA via static quenching with K_sv=2.35×10⁵L·mol^-1,the quenching rate constant K_q=2.35×10¹³ L·mol^-1·s^-1,the binding constant K_a=2.14×10¹³L·mol^-1,the binding site n=2.37.Furthermore,the proliferation inhibition effect of the complex on the cells A549,Hela and HepG2 was studied.