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Enrichment of free amino acid content and reduction of astringent taste compounds in soybean by high hydrostatic pressure
Authors:Shigeaki Ueno  Yuki Kawaguchi  Yuka Oshikiri  Hsiuming Liu  Reiko Shimada
Institution:1. Faculty of Education, Saitama University, Saitama City, Japanshigeakiu@mail.saitama-u.ac.jp;3. Faculty of Education, Saitama University, Saitama City, Japan;4. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan
Abstract:ABSTRACT

We investigated the effect of high hydrostatic pressure (HHP) on the distribution of free amino acids and isoflavones in soybean immediately after pressure treatment (100–600?MPa, 10–60?min). HHP-treatment at 200 and 300?MPa resulted in high accumulation of free amino acids. Additionally, Gly, Val, and Pro levels increased even after HHP of 400?MPa. The application of HHP-treatment to soybean decreased sources of an astringent taste such as genistein-, daizein-, and glycitein-type molecules under all pressure conditions over 200?MPa. High accumulation of free amino acids with specific pressure–time conditions would be caused by the acceleration of proteolysis and specific amino acid metabolism. We concluded that HHP alone with no subsequent storage enabled the enrichment of specific amino acids such as GABA, Glu, Lys, and Pro in soybean. Soybean with enrichment of these amino acids and improved taste should be an innovative component of functional foods.
Keywords:Soybean  amino acid  isoflavone  high hydrostatic pressure
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