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研究了氮氧源大气压等离子体射流对表面大肠杆菌灭活作用,分析了氮氧源大气压等离子体射流的光谱性质.结果表明,在放电电压为6.8kV,气体流速为4L?min-1,处理3min时,氮氧比为1:4的大气压等离子体射流对大肠杆菌的灭活率达到98.4%,接近氧气源大气压等离子体射流灭菌效果.通过大气压等离子体射流发射光谱(OES)分析了氮氧源大气压等离子体射流中活性物质,进而解释大肠杆菌微生物灭活机理,认为NO-γ、OI、·OH等活性物质在表面大肠杆菌灭活过程中起到了重要作用.这将为大气压氮氧等离子体射流在环境卫生、微生物灭活等方面的应用研究提供实验基础和技术支持.

Spectrum analysis and surface inactivation of E. coli bacteria by atmospheric pressure N2/O2 plasma jet

The sterilization effects of surface E. coli bacteria by N2/O2 source atmospheric pressure plasma jet (APPJ) were studied, and the spectroscopic properties of nitrogen/oxygen source atmospheric pressure plasma jet were analyzed. Experimental results showed that under discharge voltage at 6.8 kV, gas flow rate at 4L?min?1 and treatment time at 3min, E. coli bacteria inactivation rate of APPJ with N2/O2 at 1:4 reached 98.4%, close to that of oxygen source APPJ. Active substances of nitrogen/oxygen source plasma jet were analyzed by optical emission spectroscopy (OES) to interpret the mechanism of the microorganism of E. coli bacteria, and it was considered that active substances, such as NO?γ, OI, ?OH, played the important roles in the surface E. coli bacteria inactivation process. This would provide the experimental basis and technical support of application researches of atmospheric pressure N2/O2 plasma jet, such as environmental hygiene, microbial inactivation and so on.