Abstract: | We present results of Brillouin light scattering studies of lysozyme and guanidine hydrochloride solutions in the temperature range 290–350 K. The Brillouin spectra of 6 M guanidine hydrochloride have been found to contain an additional component in Rayleigh scattering that manifests itself as a broad quasi‐elastic scattering line centered at the unshifted frequency and described by a Lorentz function (i.e. a Debye relaxor with relaxation time τ1 ~ 25 ps at room temperature). The temperature dependence of τ1 is described by the Arrhenius law with activation energy Ea = 0.11 ± 0.01 eV and prefactor τ0= 0.33 ± 0.03 ps. The Brillouin spectra of lysozyme denatured by 6 M guanidine hydrochloride exhibit a more complicated structure of the additional contribution into Rayleigh scattering, which is fitted best of all by a sum of two Lorentzians centered at the unshifted frequency (with relaxation times τ1 ~ 19 ps and τ2 ~ 180 ps at 339 K). Possible origins of the quasi‐elastic scattering are discussed. Copyright © 2012 John Wiley & Sons, Ltd. |