Fluorometric and Derivative Spectrophotometric Determination of Norfloxacin

The method for the direct determination of norfloxacin, without prior separation, in serum and pharmaceutical formulations, by means of fluorometry and second derivative u.v. spectrophotometry, was developed. Fluorometric assay of norfloxacin in serum was carried in 0.1 M HCl, with the adition of sodium-dodecylsulphate, at emission wavelength 450 nm (excitation 320 nm). Linear calibration curve was obtained in the concentration range 20 – 320 μg/L with the detection limit 2μg/L. The second derivative spectrophotometry was used for the determination of the norfloxacin in tablets at 337 nm using 0.05 M NaOH as solvent. Detection limit was 30μg/L.