Influence of Lanolin and White Soft Paraffin on the Determination of Vitamin a in Fats |
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| Authors: | M S Jovanović M Gutalj D Ivanović |
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| Institution: | 1. Laboratory for Radioisotopes , Vinca Institute of Nuclear Sciences , P. O. Box 522, 11000, Belgrade, Yugoslavia;2. ICN Jugoslavia , Quality Control, 29 Novembar 111, 11000, Belgrade, Yugoslavia;3. Department of Pharmaceutical Chemistry and Drug Analysis , Faculty of Pharmacy , Vojvode Stepe 450, 11000, Belgrade, Yugoslavia |
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| Abstract: | Pharmaceutical interaction of the most frequently used substances with fats: lanolin, white soft paraffin and vitamin A palmitate was studied. The mixtures were examined by TLC with different ratios of lanolin and vitamin A palmitate, and white soft paraffin and vitamin A palmitate. Vitamin A in the mixtures was determined by the UV spectrophotometric method according to BP 93. It was established that the rise of lanolin concentration resulted in an increased apparent content of vitamin A in fats as a consequence of lanolin absorption at the wave length of maximum absorption of vitamin A. By an increase of white soft paraffin concentration, an apparent decreased in the content of vitamin A in fats was obtained. Therefore, the suggested UV spectrophotometric method for vitamin A determination has a limited application on fats with high content of lanolin and white soft paraffin. |
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| Keywords: | UV spectrophotometry vitamin A palmitate anhydrous lanolin white soft paraffin pharmaceutical interaction |
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