In Vivo Temporal EPR Measurements in the Lung of Mice by a Selected-Region Intensity Determination Method |
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Authors: | H Yokoyama T Ono |
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Institution: | (1) National Institute of Advanced Industrial Science and Technology, Nagoya, Japan |
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Abstract: | Selected-region intensity determination (SRID) is a method for obtaining the temporal changes in electron paramagnetic resonance
(EPR) signal intensity from a selected region without complicated procedures used in the previous imaging method. We used
an in vivo 700 MHz radiofrequency EPR spectrometer equipped with a bridged loop-gap resonator and the SRID method to perform
temporal EPR measurements of the lung area of mice which had received a nitroxide radical (3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl)
administration via the intravenous route. The half-life and initial level of nitroxide radical in the lung or the mediastinum
were calculated from temporal changes in the signal intensity. A mathematical model was devised to determine the nitroxide
radical concentration in the lung, which is connected to other organs via the circulatory system. Using this model and the
results of the EPR measurements, the degrees of influence of the nitroxide reduction in the lung and other organs were simulated.
It was found that the reaction rate (=log2/half-life) obtained from the lung mainly reflected the reduction of nitroxide radical
there.
Authors' address: Hidekatsu Yokoyama, National Institute of Advanced Industrial Science and Technology, 2266-98 Anagahora,
Shimoshidami, Moriyama-ku, 463-8560 Nagoya, Japan |
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Keywords: | |
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