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High speed measurement of corn seed viability using hyperspectral imaging
Institution:1. Department of Biosystems Machinery Engineering, College of Agricultural and Life Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea;2. Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Powder Mill Rd. Bldg. 303, BARC-East, Beltsville, MD 20705, USA;1. Department of Food Science, Stellenbosch University, Private Bag X1, Matieland (Stellenbosch) 7602, South Africa;2. Department of Chemistry and Bioscience, Aalborg University, Esbjerg, Denmark;1. Center for Analytical Instrumentation Development, Korea Basic Science Institute, 169-148 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea;2. Department of Biosystems Machinery Engineering, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 305-764, Republic of Korea;3. Department of Horticultural Bioscience, Pusan National University, 50 Samnangjin-eup, Miryang 627-706, Republic of Korea;1. Key Laboratory of Electronic Engineering of Heilongjiang Province, Heilongjiang University, Harbin 150080, China;2. Key Laboratory of Sugarbeet Genetics and Breeding, Heilongjiang University, Harbin 150080, China;1. Key Laboratory of Advanced Process Control for Light Industry (Ministry of Education), Jiangnan University, Wuxi 214122, China;2. Department of Electrical & Computer Engineering, Oakland University, USA;1. Key Laboratory of Electronic Engineering of Heilongjiang Province (Heilongjiang University), Harbin 150080, China;2. Heilongjiang Provincial Crop Research Institute (Heilongjiang University), Harbin 150080, China
Abstract:Corn is one of the most cultivated crops all over world as food for humans as well as animals. Optimized agronomic practices and improved technological interventions during planting, harvesting and post-harvest handling are critical to improving the quantity and quality of corn production. Seed germination and vigor are the primary determinants of high yield notwithstanding any other factors that may play during the growth period. Seed viability may be lost during storage due to unfavorable conditions e.g. moisture content and temperatures, or physical damage during mechanical processing e.g. shelling, or over heating during drying. It is therefore vital for seed companies and farmers to test and ascertain seed viability to avoid losses of any kind. This study aimed at investigating the possibility of using hyperspectral imaging (HSI) technique to discriminate viable and nonviable corn seeds. A group of corn samples were heat treated by using microwave process while a group of seeds were kept as control group (untreated). The hyperspectral images of corn seeds of both groups were captured between 400 and 2500 nm wave range. Partial least squares discriminant analysis (PLS-DA) was built for the classification of aged (heat treated) and normal (untreated) corn seeds. The model showed highest classification accuracy of 97.6% (calibration) and 95.6% (prediction) in the SWIR region of the HSI. Furthermore, the PLS-DA and binary images were capable to provide the visual information of treated and untreated corn seeds. The overall results suggest that HSI technique is accurate for classification of viable and non-viable seeds with non-destructive manner.
Keywords:Hyperspectral imaging  Corn seed  Viability  PLS-DA  Image processing
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