In vivo imaging of dynamic biological specimen by real-time single-shot full-field optical coherence tomography |
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Authors: | Molly Subhash Hrebesh Razvan Dabu |
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Institution: | a Graduate School of Human Sensing and Functional Sensor Engineering, Graduate School of Science and Engineering, Yamagata University, 4-3-16 Johnan, Yonezawa 992-8510, Japan b National Institute for Laser, Plasma and Radiation Physics, MG-36, Bucharest 077125, Romania |
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Abstract: | We demonstrate the feasibility of a compact single-shot full-field time domain optical coherence tomography (OCT) for imaging dynamic biological sample in real-time. The system is based on a Linnik type polarization Michelson interferometer and a four-quadrature phase-stepper optics, which can simultaneously capture four quadraturely phase-stepped interferograms on a single CCD. Using a superluminescent diode as light source with center wavelength of 842 nm and spectral width of 16.2 nm, the system yields an axial resolution of 19.8 μm, and covers a field of view of 280 × 320 μm2 (220 × 250 pixels) with a transverse resolution of 4.4 μm by using a 10× microscope objective (0.3 NA). Three-dimensional OCT images of biological samples such as an onion slice and a diaptomus were obtained without any image averaging or pixel binning. In addition, in vivo depth resolved dynamic imaging was demonstrated to show the beating internal structure of a diaptomus with a fame rate of 5 fps. |
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Keywords: | Optical coherence tomography Medical and biological imaging Medical optics instrumentation Three-dimensional image processing Interferometry |
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