Design, simulation and characterisation of integrated optics for a microfabricated flow cytometer |
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Authors: | David Barat Giuseppe Benazzi Jesus Miguel Ruano |
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Institution: | a School of Electronics and Computer Science, University of Southampton, Highfield, Southampton SO17 1BJ, UK b National Oceanography Centre, University of Southampton Waterfront Campus, European Way, Southampton S014 3ZH, UK c MEMS/MST Department, IKERLAN S. Koop., Paseo J.M. Arizmendiarrieta No. 2, Arrasate 20500, Spain |
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Abstract: | Flow cytometry is widely used for analyzing micro-particles such as cells and bacteria. Microfabricated flow cytometers promise reduced instrument size and cost with increased robustness and have application in medicine, life sciences and environmental metrology. Further miniaturisation and robustness can be achieved if integrated optics are used instead of traditional free space optics. We present designs simulation and experimental characterisation of integrated optics for a microfabricated cytometer made from SU-8 resin on a glass substrate. The optics constructed from combinations of optical fibres (positioned with microgrooves), waveguides, and microlenses enable analysis of scattered light and fluorescence from particles positioned near the centre of a microchannel using one dimensional sheath flow. Four different methods for directing the incident light onto the particles are examined and the optimum design discussed. |
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Keywords: | Integrated optics Flow cytometry Optical losses Collimation of light |
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