Visible-super-resolution infrared microscopy using saturated transient fluorescence detected infrared spectroscopy |
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Authors: | Nándor Bokor Keiichi Inoue Masaaki Fujii |
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Institution: | a Department of Physics, Budapest University of Technology and Economics, 1111 Budapest, Budafoki, u. 8, Hungary b Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midoriku, Yokohama 226-8503, Japan |
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Abstract: | A scanning visible-super-resolution microscope based on the saturation behaviour of transient fluorescence detected infrared (TFD-IR) spectroscopy is proposed. A Gaussian IR beam, a Gaussian visible beam and a Laguerre-Gaussian (LG) visible beam are used to obtain two separate two-color excitation fluorescence (2CF) images of the sample. The final image is obtained as the difference between the two recorded images. If the peak intensity of the LG beam is high enough to induce saturation in the fluorescence signal, the image can, in principle, have unlimited spatial resolution. A ∼3-fold improvement in transverse resolution over the visible diffraction limit (and far exceeding the IR diffraction limit) is easily achievable in present experimental setups. |
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Keywords: | 130 3060 180 0180 180 2520 |
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