| 活细胞内的单分子荧光成像方法 |
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| 引用本文： | 林丹樱,马万云.活细胞内的单分子荧光成像方法[J].物理,2007,36(10):783-790. |
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| 作者姓名： | 林丹樱 马万云 |
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| 作者单位： | 清华大学物理系原子分子纳米科学教育部重点实验室,北京,100084 |
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| 基金项目： | 国家自然科学基金;教育部科学技术研究项目 |
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| 摘 要： | 文章介绍近年来新发展的几种重要的活细胞内单分子荧光成像方法,如转盘式共聚焦显微术、全内反射荧光显微术、荧光共振能量转移技术等。通过介绍它们的原理、特点和在活细胞内单分子行为研究中的应用实例,展示了这些新方法在生命科学领域广阔的应用前景。
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| 关 键 词： | 单分子 荧光 成像 活细胞 |
| 修稿时间： | 2007-06-19 |
Single molecule fluorescence imaging within living cells |
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| LIN Dan-Ying,MA Wan-Yun.Single molecule fluorescence imaging within living cells[J].Physics,2007,36(10):783-790. |
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| Authors: | LIN Dan-Ying MA Wan-Yun |
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| Institution: | The Key Laboratory of Atoraic and Molecular Nanosciences, Ministry of Education, Tsinghua University, Beijing 100084, China |
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| Abstract: | Single molecule detection techniques based on fluorescence imaging such as spinning disk confocal microscopy, total internal reflection fluorescence microscopy and fluorescence resonance energy transfer are reviewed. These techniques have been used in recent years as useful tools to study single molecule behavior within living cells and show broad prospects for application in the life sciences. |
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| Keywords: | single molecule fluorescence imaging living cells |
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