Targeted destruction of murine macrophage cells with bioconjugated gold nanorods |
| |
| Authors: | Dakrong Pissuwan Stella M Valenzuela Murray C Killingsworth Xiaoda Xu Michael B Cortie |
| |
| Institution: | (1) Institute for Nanoscale Technology, University of Technology Sydney, P.O. Box 123, Broadway, NSW, 2007, Australia;(2) Department of Medical and Molecular Biosciences, University of Technology Sydney, P.O. Box 123, Broadway, NSW, 2007, Australia;(3) Sydney South West Pathology Service, Locked Bag 7090, Liverpool BC, NSW, 1871, Australia |
| |
| Abstract: | Gold nanorods manifest a readily tunable longitudinal plasmon resonance with light and consequently have potential for use
in photothermal therapeutics. Recent work by others has shown how gold nanoshells and rods can be used to target cancer cells,
which can then be destroyed using relatively high power laser radiation (∼1×105 to 1×1010 W/m2). Here we extend this concept to demonstrate how gold nanorods can be modified to bind to target macrophage cells, and show
that high intensity laser radiation is not necessary, with even 5×102 W/m2 being sufficient, provided that a total fluence of ∼30 J/cm2 is delivered. We used the murine cell line RAW 264.7 and the monoclonal antibody CD11b, raised against murine macrophages,
as our model system and a 5 mW solid state diode laser as our energy source. Exposure of the cells labeled with gold nanorods
to a laser fluence of 30 J/cm2 resulted in 81% cell death compared to only 0.9% in the control, non-labeled cells. |
| |
| Keywords: | hyperthermal therapy plasmon resonance plasmonic heating active and passive targeting biomedicine nanobiotechnology |
| 本文献已被 SpringerLink 等数据库收录！ |
|
