Phosphorylcholine functionalized dendrimers for the formation of highly stable and reactive gold nanoparticles and their glucose conjugation for biosensing |
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Authors: | Lan Jia Li-Ping Lv Jian-Ping Xu Jian Ji |
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Institution: | (1) Department of Polymer Science and Engineering, MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Zhejiang University, Hangzhou, 310027, China; |
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Abstract: | Phosphorylcholine (PC)-functionalized poly(amido amine) (PAMAM) dendrimers were prepared and used as both reducing and stabilizing
agents for synthesis of highly stable and reactive gold nanoparticles (Au NPs). Biomimetic PC-functionalized PAMAM dendrimers-stabilized
gold nanoparticles (Au DSNPs) were formed by simply mixing the PC modified amine-terminated fifth-generation PAMAM dendrimers
(G5-PC) with AuCl4
− ions by controlling the pH, no additional reducing agents or other stabilizers were needed. The obtained Au DSNPs were shown
to be spherical, with particle diameters ranging from 5 to 12 nm, the sizes and growth kinetics of Au DSNPs could be tuned
by changing the pH and the initial molar ratio of dendrimers to gold as indicated by transmission electron microscopy (TEM)
and UV–Vis data. The prepared Au DSNPs showed excellent stability including: (1) stable at wide pH (7–13) values; (2) stable
at high salt concentrations up to 2 M NaCl; (3) non-specific protein adsorption resistance. More importantly, surface functionalization
could be performed by introducing desired functional groups onto the remained reactive amine groups. This was exemplified
by the glucose conjugation. The glucose conjugated Au DSNPs showed bio-specific interaction with Concanavalin A (Con A), which
induced aggregation of the Au NPs. Colorimetric detection of Con A based on the plasmon resonance of the glucose conjugated
Au DSNPs was realized. A limit of detection (LOD) for Con A was 0.6 μM, based on a signal-to-noise ratio (S/N) of 3. These
findings demonstrated that the PC modified Au DSNPs could potentially serve as a versatile nano-platform for the biomedical
applications. |
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Keywords: | |
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