Controlled self assembly of collagen nanoparticle |
| |
Authors: | Massimiliano Papi Valentina Palmieri Giuseppe Maulucci Giuseppe Arcovito Emanuela Greco Gianluca Quintiliani Maurizio Fraziano Marco De Spirito |
| |
Institution: | 1.Università Cattolica del Sacro Cuore, Istituto di Fisica,Rome,Italy;2.Department of Biology,University of Rome ‘‘Tor Vergata”,Rome,Italy |
| |
Abstract: | In recent years carrier-mediated drug delivery has emerged as a powerful methodology for the treatment of various pathologies.
The therapeutic index of traditional and novel drugs is enhanced via the increase of specificity due to targeting of drugs
to a particular tissue, cell or intracellular compartment, the control over release kinetics, the protection of the active
agent, or a combination of the above. Collagen is an important biomaterial in medical applications and ideal as protein-based
drug delivery platform due to its special characteristics, such as biocompatibility, low toxicity, biodegradability, and weak
antigenicity. While some many attempts have been made, further work is needed to produce fully biocompatible collagen hydrogels
of desired size and able to release drugs on a specific target. In this article we propose a novel method to obtain spherical
particles made of polymerized collagen surrounded by DMPC liposomes. The liposomes allow to control both the particles dimension
and the gelling environment during the collagen polymerization. Furthermore, an optical based method to visualize and quantify
each step of the proposed protocol is detailed and discussed. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|