Regulation of catalytic behaviour of hydrolases through interactions with functionalized carbon-based nanomaterials |
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Authors: | Ioannis V Pavlidis Torge Vorhaben Dimitrios Gournis George K Papadopoulos Uwe T Bornscheuer Haralambos Stamatis |
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Institution: | (1) Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, 45110 Ioannina, Greece;(2) Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany;(3) Department of Materials Science & Engineering, University of Ioannina, 45110 Ioannina, Greece;(4) Laboratory of Biochemistry and Biophysics, Faculty of Agricultural Technology, Epirus Institute of Technology, 47110 Arta, Greece; |
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Abstract: | The interaction of enzymes with carbon-based nanomaterials (CBNs) is crucial for the function of biomolecules and therefore
for the design and development of effective nanobiocatalytic systems. In this study, the effect of functionalized CBNs, such
as graphene oxide (GO) and multi-wall carbon nanotubes (CNTs), on the catalytic behaviour of various hydrolases of biotechnological
interest was monitored and the interactions between CBNs and proteins were investigated. The enzyme–nanomaterial interactions
significantly affect the catalytic behaviour of enzymes, resulting in an increase up to 60 % of the catalytic efficiency of
lipases and a decrease up to 30 % of the esterase. Moreover, the use of CNTs and GO derivatives, especially those that are
amine-functionalized, led to increased thermal stability of most the hydrolases tested. Fluorescence and circular dichroism
studies indicated that the altered catalytic behaviour of enzymes in the presence of CBNs arises from specific enzyme–nanomaterial
interactions, which can lead to significant conformational changes. In the case of lipases, the conformational changes led
to a more active and rigid structure, while in the case of esterases this led to destabilization and unfolding. Kinetic and
spectroscopic studies indicated that the extent of the interactions between CBNs and hydrolases can be mainly controlled by
the functionalization of nanomaterials than by their geometry. |
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