Sectional image reconstruction and three-dimensional microscopy of small particles by angular spectrum method |
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Authors: | Naseem Akhter Chang-Doo Kee Man-Yong Choi |
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Institution: | a Department of Mechanical Design Engineering, Chosun University, 375 Seoseok-Dong, Dong-Gu, Gwangju 501-759, Republic of Korea b School of Mech. System Engineering, Chonnam National University, 300 Yongbong-Dong, Buk-Gu, Gwangju, 500-757, Republic of Korea c Korea Research Institute of Standards and Science (KRISS), P.O. Box 102, Yuseong, Daejeon 305-600, Republic of Korea |
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Abstract: | In this letter, we demonstrate sectional image reconstruction and three-dimensional microscopy of small particles. We demonstrate sectional image reconstruction and holographic methods to obtain 2D and 3D images of small particles. A single hologram is sufficient to obtain a section containing only the focused parts of the reconstructed image. One can obtain images of different plane sections of a specimen in addition to its 3D display. The reconstruction of a digital hologram is based on the plane-wave expansion of the diffracted wave fields using Fourier optics (this method is also known as the angular spectrum method). With this method, the object-to-hologram distance can be quite small because the minimum-distance requirement does not apply. Furthermore, numerical reconstruction of transparent objects by this method may be interesting for micro-structure measurement. |
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Keywords: | Digital holography 3D microscopy Image reconstruction Fourier optics |
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