| 人肝癌SMMC-7721细胞对低剂量γ射线辐射超敏感性的研究 |
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| 引用本文： | 金晓东,李强,李文建,王菊芳,郭传玲,郝冀芳.人肝癌SMMC-7721细胞对低剂量γ射线辐射超敏感性的研究[J].原子核物理评论,2005,22(2):208-211. |
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| 作者姓名： | 金晓东 李强 李文建 王菊芳 郭传玲 郝冀芳 |
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| 作者单位： | [1]中国科学院近代物理研究所,甘肃兰州730000 [2]中国科学院近代物理研究所,甘肃兰州730000//中国科学院研究生院,北京100039 |
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| 基金项目： | 国家自然科学基金资助项目(10205021)~~ |
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| 摘 要： | 研究了人肝癌细胞SMMC-7721在低剂量γ射线照射下超敏感性和增强的辐射抗性响应。选用对数生长期细胞接受0—6 Gy不同剂量的^60Coγ射线的照射。利用流式细胞仪对细胞进行分选计数，并用克隆形成法检测细胞存活率。发现SMMC-7721细胞存在低剂量辐射超敏感性和增强的辐射抗性响应，即在0—0．3Gy之间细胞表现出单位剂量杀伤增强现象，在0．3—1Gy细胞表现一定的辐射抗性，在1Gy以上，细胞的存活符合线性平方模型。
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| 关 键 词： | 辐射超敏感性 增强的辐射抗性 细胞克隆形成率 细胞存活分数 |
| 文章编号： | 1007-4627(2005)02-0208-04 |
| 收稿时间： | 1900-01-01 |
| 修稿时间： | 2004年11月1日 |
Study on Hyper-radiosensitivity Effect of Human Hepatoma SMMC-7721 Cells Exposed to Low Dose γ-rays |
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| JIN Xiao-dong,LI Qiang,LI Wen-jian,WANG Ju-Fang,GUO Chuan-Ling,HAO Ji-fang.Study on Hyper-radiosensitivity Effect of Human Hepatoma SMMC-7721 Cells Exposed to Low Dose γ-rays[J].Nuclear Physics Review,2005,22(2):208-211. |
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| Authors: | JIN Xiao-dong LI Qiang LI Wen-jian WANG Ju-Fang GUO Chuan-Ling HAO Ji-fang |
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| Institution: | (1 Institute of Modern Physics; Chinese Academy of Sciences; Lanzhou 730000; China; 2 Graduate School of Chinese Academy of Sciences; Beijing 100039; China); |
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| Abstract: | The low-dose responses such as hypersensitivity and increased radio-resistance of human hepatoma SMMC-7721 cells were studied in this work. Exponentially growing SMMC-7721 cells were irradiated with ~(60)Co γ-rays at doses of 0—6 Gy. Together with flow cytometer for precise cell sorting, cell survival fractions were measured by means of conventional colony-formation assay. It has been found that the SMMC-7721 cells showed hyper-radiosensitivity response at low doses and increased radio-resistance at larger |
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| Keywords: | hypersensitivity increased radio-resistance colony-formation assays survival fraction |
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