GANRA类药物的抗辐射效应研究

以昆明小鼠为实验模型， 连续进行GANRA类药物灌胃3 d后， 使用8 Gy 100 kVp的X射线进行辐照处理， 然后对血象、 脏器指数、 小鼠存活率、 肝脏中丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性等参数进行了统计分析。 结果表明: (1) 药物灌胃对小鼠存活率没有影响。 辐照后， 小鼠存活率显著下降， 存活率下降至50%所需时间分别为: 照射对照组20 d、 DMSO组9 d、 1#药物组29 d、 5#药物组24 d; (2) 药物灌胃对小鼠脏器指数没有明显影响, 辐照后的对照组和DMSO组小鼠的肝脏指数、 脾脏指数升高， 药物组的脏器指数低于照射对照组和DMSO组; (3) 辐照导致各实验组的MDA水平升高、 SOD活性下降， 但是5#药物的SOD活性高于DMSO组、 MDA水平低于DMSO组; (4) 药物灌胃对小鼠血象没有产生明显毒性效应；辐照后各实验组小鼠的白细胞等都显著下降。 从上述实验结果可以看出， GANRA类1#和5#药物具有抗辐射作用， 其机制可能与GANRA类药物的自由基清除能力有关。 After three days of medicine administrated, Kunming mice were exposured to 8 Gy of 100 kVp X rays. Hemogram, viscere, survival, MDA yields and SOD activity were examined. Results showes that, (1) Survival of medicine administrated mice were similar to the control group. After irradiation, survival dropped significantly. It took 20 days for the control group to reach to 50% survival, while 9 days for DMSO group 29 days for 1# drug group, and 24 days for 5# drug group. (2) The medicine per se had no obvious impacts on visceral indexes. Liver index and spleen index of the control group and DMSO group were elevated after irradiated, while those of medicine administrated group did not change much. (3) The groups treated by DMSO and 1# drug had high yield of MDA, but low activity of SOD. Compared with DMSO group, 5# drug had high SOD activity while low MDA level. Irradiation increased MDA level but decreased SOD activity of every group, However, 5# drug still showed higher SOD activity and lower MDA level than DMSO group. (4) The medicine did not show distinctive contributions to variation of hemogram. In summary, our results demonstrated that GANRA like 1# and 5# medicine had radioprotective effects and their mechanisms might be related to the scavenging ability of free radicals.

Study on Radioprotective Effects of GANRA-like Medicine

After three days of medicine administrated,Kunming mice were exposured to 8 Gy of 100 kVp X-rays.Hemogram,viscere,survival,MDA yields and SOD activity were examined.Results showes that,(1) Survival of medicine-administrated mice were similar to the control group.After irradiation,survival dropped significantly.It took 20 days for the control group to reach to 50% survival,while 9 days for DMSO group 29 days for 1# drug group,and 24 days for 5# drug group.(2) The medicine per se had no obvious impacts on visceral indexes.Liver index and spleen index of the control group and DMSO group were elevated after irradiated,while those of medicine-administrated group did not change much.(3) The groups treated by DMSO and 1# drug had high yield of MDA,but low activity of SOD.Compared with DMSO group,5# drug had high SOD activity while low MDA level.Irradiation increased MDA level but decreased SOD activity of every group,However,5# drug still showed higher SOD activity and lower MDA level than DMSO group.(4) The medicine did not show distinctive contributions to variation of hemogram.In summary,our results demonstrated that GANRA-like 1# and 5# medicine had radioprotective effects and their mechanisms might be related to the scavenging ability of free radicals.