超分辨率成像荧光探针材料应用进展

为了进一步认知复杂环境中的细胞生物学过程,研究人员发展了各种各样的生物成像技术。在这些技术中,生物荧光成像因简单的成像条件以及对生物样品的相容性而得到了广泛的发展。然而,传统的荧光成像技术受到了光学衍射极限的限制,无法分辨低于200 nm的空间结构,阻碍了对亚细胞结构的生物学过程研究。超分辨荧光显微镜技术突破了传统光学衍射对成像分辨率的限制,能够获取纳米尺度的细胞动态过程。除了对传统的宽场荧光显微镜框架的改进及升级改造之外,目前典型的超分辨成像显微镜技术通常依赖于荧光探针材料的光物理性质。常用的荧光探针材料包括荧光蛋白、有机荧光分子和纳米荧光材料等。本文介绍了几种主流的超分辨荧光显微成像技术并总结了已经成功应用到超分辨生物荧光成像中的荧光探针材料的应用进展。

Advances in application of materials of super-resolution imaging fluorescent probe

In order to further understand the biological cellular processes in the complex environments, a variety of bioimaging techniques have been developed by researchers. Biofluorescence imaging has been extensively developed due to its simple imaging conditions and compatibility with biological samples. However, the traditional fluorescence imaging technology is restricted by the optical diffraction limit, so it is impossible to resolve the spatial structure below 200 nm, which hinders the study of the biological processes of subcellular structures. Super-resolution fluorescence microscopy breaks through the limitations of imaging resolution with traditional optical diffraction and can acquire nanoscale cellular dynamics. In addition to improvements and upgrades to traditional wide-field fluorescence microscope frames, typical super-resolution imaging microscopy techniques currently also rely on the photophysical properties of fluorescent probe materials. Commonly used fluorescent probe materials mainly include fluorescent proteins, organic fluorescent molecules and fluorescent nanomaterials. This paper introduces several mainstream super-resolution fluorescence microscopy techniques and summarizes the application status of fluorescent probe materials that have been successfully applied to super-resolution biofluorescence imaging.