微流控SERS芯片及其生物传感应用

由于微流控芯片具有优异的集成性和灵活的可操作性，基于芯片上的检测方法被大量开发，发展十分迅速。其中，表面增强拉曼光谱（SERS）凭借其超高的灵敏度、独一无二的指纹谱和窄峰宽等特点成为一种广泛采用的检测手段。SERS微流控芯片集SERS检测技术与微流控芯片的优势于一体，一方面为SERS检测方法的重复性和可靠性提供了一个高效平台，另一方面推动了微流控芯片的功能拓展，在生物分子探测、细胞捕获乃至组织模拟等领域具有广阔的应用前景。本文在简要介绍SERS的原理及其生物传感应用的基础上，重点概述了SERS微流控芯片的构建及其在生物传感及检测中的应用，最后探讨了该研究方向存在的问题及发展方向。

Microfluidic SERS chip and its biosensing applications

With excellent integrability and flexible operability, microfluidic chips have been developed rapidly. Among them, Surface-enhanced Raman Spectroscopy (SERS) has become a widely used detection technique due to its ultrasensitivity, unique fingerprint spectrum and narrow spectroscopic bands. The SERS microfluidic chip integrates the advantages of the SERS detection technology and the microfluidic chip. On the one hand, it provides an efficient platform for the repeatability and reliability of the SERS detection method. On the other hand, it promotes function expansion for microfluidic chips. The SERS microfluidic chips have broad application prospects in the fields of biomolecule detection, cell capture and even tissue simulation. In this review, the principle of SERS is briefly introduced, and the construction of SERS microfluidic chip and its applications in biosensing and detection are emphatically summarized. Finally, the problems and development direction of the research are proposed.