血液静态荧光偏振光谱研究

报道了用408 nm的LED偏振光诱导浓度为0.3%的人全血溶液在350800 nm波段的静态荧光偏振光谱,讨论了其光谱的结构特征和红移现象并给出了机理解释;检测了不同浓度全血溶液荧光偏振光谱中在490nm和610 nm附近处两个各主要荧光区的偏振度,实验结果表明,当血液浓度为1.0%时,在两个主要荧光区的偏振度分别为0.4796和0.4344,当浓度增加到8.0%时,相应的偏拓坟墓分别为0.2064和0.0538。研究了偏振度随浓度的变化规律,用能量转移理论分析了在不同荧光区、不同浓度下,血液中各荧光团之间的不同能量转换机理及偏振度的变化现象。研究结果对光诱导生物组织自体荧光诊断技术有一定的参考价值。

Research on Static Fluorescence Polarization Spectrum of Whole Blood

The) static fluorescence polarization spectrum of human whole blood whose concentrations are from 0.3% to 10% at the band 350\~800 nm induced by 408 nm polarized light of LED is reported.The spectral characteristics and red-shifted phenomenon for different concentrations of the sample solutions are discussed,and the mechanism of the red shift is also given.The polarization degrees of two key fluorescence emission bands(around 490 nm and 610 nm) in polarization spectrum are measured.The result shows that the polarization degrees of two key flourescence emission bands are 0.4796 and 0.4344 when the concentration of blood is 1.0%,and the polarization degrees are 0.2064 and 0.0538 when the concentration is 8.0%.The variation rule of the polarization degree with the concentrations of whole blood is studied.The different energy transfer mechanisms as well as the variation of polarization degree in different fluorescence areas for different concentrations of whole blood is studied by the theory of energy transfer.The research will gives reference to light-induced autofluorescence diagnostic techniques of organic tissue.