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| 光谱学研究血卟啉与鲱鱼精DNA的相互作用 | |
| 引用本文： | 黎泓波,拓宏桂,王兴明,王素琴,丁力生.光谱学研究血卟啉与鲱鱼精DNA的相互作用[J].光学学报,2008,28(10):2015-2021. |
| 作者姓名： | 黎泓波 拓宏桂 王兴明 王素琴 丁力生 |
| 作者单位： | 1. 龙岩学院 化学与材料学院,福建 龙岩 364000;西南科技大学材料科学与工程学院,四川,绵阳,621010 2. 龙岩学院 化学与材料学院,福建 龙岩,364000 3. 西南科技大学材料科学与工程学院,四川,绵阳,621010 4. 中南大学,化学化工学院,湖南,长沙,410083 5. 中国科学院成都生物研究所,四川,成都,610041 |
| 摘 要： | 以中性红(NR)作探针研究了血卟啉(HD)与DNA的相互作用.光谱学研究表明,血卟啉与DNA发生作用生成了超分子复合物.其结合比nHD:nDNA=3:1,血卟啉与DNA的结合常数K=1.84×104L/mol.同时运用Scatchart方程研究了血卟啉与DNA的作用方式,确定了血卟啉与鲱鱼精DNA之间的作用方式为混合作用方式.其作用机理可能是血卟啉以部分插入方式同时伴随共价和静电方式与DNA作用,影响其基因调控与表达,进而抑制肿瘤细胞的生长. |
| 关 键 词： | 光谱学 血卟啉二盐酸盐 中性红 作用方式 |
| 收稿时间： | 2008/1/10 |
Study on Interaction between Hematoporphyrin Dihydrochloride and Herring Sperm DNA by Spectroscopy |
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| Li Hongbo,Ta Honggui,Wang Xingming,Wang Suqin,Ding Lisheng.Study on Interaction between Hematoporphyrin Dihydrochloride and Herring Sperm DNA by Spectroscopy[J].Acta Optica Sinica,2008,28(10):2015-2021. | |
| Authors: | Li Hongbo Ta Honggui Wang Xingming Wang Suqin Ding Lisheng |
| Institution: | Li Hongbo1,2 Ta Honggui1 Wang Xingming2 Wang Suqin3 Ding Lisheng41School of Chemistry , Materials Science,Longyan University,Longyan,Fujiang 364000,China2School of Materials Science , Engineering,Southwest University of Science , Technology,Mianyang,Sichuan 621010,China3School of Chemistry , Chemical Engineering,Central South University,Changsha,Hunan 410083,China4Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu,Sichuan 610041,China |
| Abstract: | The interaction of hematoporphyrin dihydrochloride (HD) with DNA was studied by using neutral red (NR) as a probe.The results indicate that a super-molecule complex of HD and DNA is produced, which has been confirmed by absorption and fluorescence spectra.The binary complex ratio of nHD∶nDNA is 3∶1, and the binding constant of HD interacting with DNA is 1.84×104 L/mol. The interaction of HD with DNA is studied. The results suggest that the mingling mode is the major mode for interaction between HD and herring sperm DNA. The mechanism is probably partial intercalation binding with covalent and electrostatical bindings. This interaction mode affects the distribution and expression of gene and resists the growth of cancer cells. |
| Keywords: | DNA |
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